Chain Format
 

The chain format describes a pairwise alignment that allow gaps in both sequences simultaneously. Each set of chain alignments starts with a header line, contains one or more alignment data lines, and terminates with a blank line. The format is deliberately quite dense.

Example:

 chain 4900 chrY 58368225 + 25985403 25985638 chr5 151006098 - 43257292 43257528 1
  9       1       0
  10      0       5
  61      4       0
  16      0       4
  42      3       0
  16      0       8
  14      1       0
  3       7       0
  48

  chain 4900 chrY 58368225 + 25985406 25985566 chr5 151006098 - 43549808 43549970 2
  16      0       2
  60      4       0
  10      0       4
  70
Header Lines 

  chain score tName tSize tStrand tStart tEnd qName qSize qStrand qStart qEnd id
The initial header line starts with the keyword chain, followed by 11 required attribute values, and ending with a blank line. The attributes include:
  • score -- chain score
  • tName -- chromosome (reference sequence)
  • tSize -- chromosome size (reference sequence)
  • tStrand -- strand (reference sequence)
  • tStart -- alignment start position (reference sequence)
  • tEnd -- alignment end position (reference sequence)
  • qName -- chromosome (query sequence)
  • qSize -- chromosome size (query sequence)
  • qStrand -- strand (query sequence)
  • qStart -- alignment start position (query sequence)
  • qEnd -- alignment end position (query sequence)
  • id -- chain ID

The alignment start and end positions are represented as zero-based half-open intervals. For example, the first 100 bases of a sequence would be represented with start position = 0 and end position = 100, and the next 100 bases would be represented as start position = 100 and end position = 200. When the strand value is "-", position coordinates are listed in terms of the reverse-complemented sequence.

Alignment Data Lines

Alignment data lines contain three required attribute values: 

  size dt dq
  • size -- the size of the ungapped alignment
  • dt -- the difference between the end of this block and the beginning of the next block (reference sequence)
  • dq -- the difference between the end of this block and the beginning of the next block (query sequence)

NOTE: The last line of the alignment section contains only one number: the ungapped alignment size of the last block.