Report for Auto_user_IONAS-289-DrSpilian_160524_ChIP_BSC1-4_387

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]9,598.36
‣ Number of Q20 Bases [Mbp]7,846.59
Total Number of Reads74,230,401
Mean Length [bp]129
Longest Read [bp]371

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]6,169.994,995.62
Mean Length [bp]11496
Longest Alignment [bp]313300
Mean Coverage Depth2.26×1.83×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C70%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,348,487
Avg Q17 read length67
50AQ17944,186

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 133,672,733 90%
Live ISPs132,722,168 99%
Test Fragment ISPs1,788,065 1%
Library ISPs130,934,103 99%
CountPercentage
Library ISPs / Percent Enrichment130,934,103 99%
Filtered: Polyclonal47,551,723 36%
Filtered: Primer dimer459,420 <1%
Filtered: Low quality8,704,233 7%
Final Library Reads74,239,795 57%

Report Information

Analysis Info

Run Name R_2016_05_24_12_18_59_user_IONAS-289-DrSpilian_160524_ChIP_BSC1-4
Run Date 2016-05-24 09:20:32+00:00
Run Flows 520
Project DrSpilianakis_ChIPseq
Sample BSC4-I
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes DrSpilianakis-4ChIPseq_BSC1-D-bc1_BSC2-S-bc2_BSC3-G-bc3_BSC4-I-bc4
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-289-DrSpilian_160524_ChIP_BSC1-4_387
Analysis Date 2016-05-24
runID INWD6

Software Version

Torrent_Suite5.0.4
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.3
ion-dbreports5.0.33-1
ion-gpu5.0.0-1
ion-pipeline5.0.16-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1