Report for Auto_user_IONAS-268-GSR9_GSkP4-5_GKLex1-4_160129_364

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]5,644.73
‣ Number of Q20 Bases [Mbp]4,098.68
Total Number of Reads70,975,203
Mean Length [bp]79
Longest Read [bp]387

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C67%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num790,059
Avg Q17 read length62
50AQ17532,270

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 128,495,776 87%
Live ISPs128,223,693 100%
Test Fragment ISPs1,202,440 <1%
Library ISPs127,021,253 99%
CountPercentage
Library ISPs / Percent Enrichment127,021,253 100%
Filtered: Polyclonal38,130,915 30%
Filtered: Primer dimer2,479,177 2%
Filtered: Low quality15,235,657 12%
Final Library Reads71,187,744 56%

Report Information

Analysis Info

Run Name R_2016_01_29_13_01_36_user_IONAS-268-GSR9_GSkP4-5_GKLex1-4_160129
Run Date 2016-01-29 11:03:05+00:00
Run Flows 520
Project DrStathopoulos_RNAseq,LexoGen_QuantSeq,DrMatis_DNA
Sample GSR9-mTECsBalbc
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes Test_Lexogen GKLex1_438-2-Sal GKLex2_438-3-Sal GKLex3_438-19-Hum GKLex4_438-23-Hum DrStath GSR9_mTECs-Balbc DrSkretas GSkP4_Ab42-Lib-Ilias GSkP5_SOD-Lib-Stefania
Barcode Set IonXpressRNA
Analysis Name Auto_user_IONAS-268-GSR9_GSkP4-5_GKLex1-4_160129_364
Analysis Date 2016-01-29
runID VAQJ1

Software Version

Torrent_Suite5.0.2
hostFW7DQV1
ion-analysis5.0.7-1
ion-chefupdates5.0.1
ion-dbreports5.0.19-1
ion-gpu5.0.0-1
ion-pipeline5.0.12-1
ion-plugins5.0.17-1
ion-protonupdates5.0.2
ion-torrentr5.0.0-1