Report for Auto_user_IONAS-326-DrStath_161220_RNAseq_GSR13-14_426

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]9,469.07
‣ Number of Q20 Bases [Mbp]7,887.48
Total Number of Reads93,380,984
Mean Length [bp]101
Longest Read [bp]386

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C93%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,076,011
Avg Q17 read length88
50AQ17998,239

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 130,675,812 88%
Live ISPs130,537,527 100%
Test Fragment ISPs1,258,749 <1%
Library ISPs129,278,778 99%
CountPercentage
Library ISPs / Percent Enrichment129,278,778 100%
Filtered: Polyclonal31,995,806 25%
Filtered: Primer dimer159,006 <1%
Filtered: Low quality4,518,748 3%
Final Library Reads93,399,171 72%

Report Information

Analysis Info

Run Name R_2016_12_21_13_27_14_user_IONAS-326-DrStath_161220_RNAseq_GSR13-14
Run Date 2016-12-21 11:29:21+00:00
Run Flows 520
Project DrStathopoulos_RNAseq
Sample GSR13-KPM3
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes DrStathopoulos_4AKRNAseq_GSR13-KPM3-bc3_GSR14-KPM4-bc4
Barcode Set IonXpressRNA
Analysis Name Auto_user_IONAS-326-DrStath_161220_RNAseq_GSR13-14_426
Analysis Date 2016-12-21
runID D9VKL

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1