for i in /data/images/proton2/run440/R_2020_02_12_12_23_36_user_IONAS-440-PMN-SPlab-DrMylonis_200212_PMNcAmp2-6_SPD5-10_IM3R7-10.SPD* 
> do
> ln -s $i
> done

reczko@max:~/Elixir/metagenomics/nr$ time ../diamond/diamond blastx --evalue 5e-6 -c 2 -b5.0  --frameshift 15 --range-culling --top 10 -d nr -q /data/images/proton2/run431/R_2019_08_07_16_07_46_user_IONAS-431-GKlab-MS_SPlab_190807_GK3R288-302_SP3R1-4.SPD3_fragDNA1.IonXpress_031.fastq -f 0 -o r431SPD3matches-longreads-top10.f0.eval5e-6 -t temp1

Total time = 4328.07s
Reported 127676609 pairwise alignments, 127676680 HSPs.
1353142 queries aligned.
real	72m8.778s

time ../diamond/diamond blastx --evalue 5e-6 -c 2 -b5.0  --frameshift 15 --range-culling --top 10 -d nr -q /data/images/proton2/run431/R_2019_08_07_16_07_46_user_IONAS-431-GKlab-MS_SPlab_190807_GK3R288-302_SP3R1-4.SPD4_fragDNA2.IonXpress_032.fastq -f 0 -o r431SPD4matches-longreads-top10.f0.eval5e-6 -t temp1

Total time = 2975.23s
Reported 90839759 pairwise alignments, 90839773 HSPs.
624324 queries aligned.
real	49m36.195s





time ../diamond/diamond blastx --evalue 5e-6 -c 2 -b5.0  --frameshift 15 --range-culling --top 10 -d nr -q /data/images/proton2/run440/R_2020_02_12_12_23_36_user_IONAS-440-PMN-SPlab-DrMylonis_200212_PMNcAmp2-6_SPD5-10_IM3R7-10.SPD10_DSM-H7.IonXpress_010.fastq -f 0 -o r440SPD10_DSM-H7-matches-longreads-top10.f0.eval5e-6 -t temp1

Total time = 1832.9s
Reported 17653712 pairwise alignments, 17653762 HSPs.
508572 queries aligned.
real	30m33.175s

time ../diamond/diamond blastx --evalue 5e-6 -c 2 -b5.0  --frameshift 15 --range-culling --top 10 -d nr -q /data/images/proton2/run440/R_2020_02_12_12_23_36_user_IONAS-440-PMN-SPlab-DrMylonis_200212_PMNcAmp2-6_SPD5-10_IM3R7-10.SPD5_shot-H4.IonXpress_005.fastq -f 0 -o r440SPD5_shot-H4-matches-longreads-top10.f0.eval5e-6 -t temp1

Total time = 5427.9s
Reported 202153119 pairwise alignments, 202153151 HSPs.
1396684 queries aligned.
real	90m28.763s

time ../diamond/diamond blastx --evalue 5e-6 -c 2 -b5.0  --frameshift 15 --range-culling --top 10 -d nr -q /data/images/proton2/run440/R_2020_02_12_12_23_36_user_IONAS-440-PMN-SPlab-DrMylonis_200212_PMNcAmp2-6_SPD5-10_IM3R7-10.SPD6_shot-H6.IonXpress_006.fastq -f 0 -o r440SPD6_shot-H6-matches-longreads-top10.f0.eval5e-6 -t temp1

Total time = 3393.88s
Reported 84227401 pairwise alignments, 84227438 HSPs.
1144092 queries aligned.
real	56m34.806s

time ../diamond/diamond blastx --evalue 5e-6 -c 2 -b5.0  --frameshift 15 --range-culling --top 10 -d nr -q /data/images/proton2/run440/R_2020_02_12_12_23_36_user_IONAS-440-PMN-SPlab-DrMylonis_200212_PMNcAmp2-6_SPD5-10_IM3R7-10.SPD7_shot-H7.IonXpress_007.fastq -f 0 -o r440SPD7_shot-H7-matches-longreads-top10.f0.eval5e-6 -t temp1

Total time = 1550.42s
Reported 10783567 pairwise alignments, 10783571 HSPs.
349691 queries aligned.
real	25m50.654s

time ../diamond/diamond blastx --evalue 5e-6 -c 2 -b5.0  --frameshift 15 --range-culling --top 10 -d nr -q /data/images/proton2/run440/R_2020_02_12_12_23_36_user_IONAS-440-PMN-SPlab-DrMylonis_200212_PMNcAmp2-6_SPD5-10_IM3R7-10.SPD8_DSM-H4.IonXpress_008.fastq -f 0 -o r440SPD8_DSM-H4-matches-longreads-top10.f0.eval5e-6 -t temp1

Total time = 2975.23s
Reported 90839759 pairwise alignments, 90839773 HSPs.
624324 queries aligned.
real	49m36.195s

time ../diamond/diamond blastx --evalue 5e-6 -c 2 -b5.0  --frameshift 15 --range-culling --top 10 -d nr -q /data/images/proton2/run440/R_2020_02_12_12_23_36_user_IONAS-440-PMN-SPlab-DrMylonis_200212_PMNcAmp2-6_SPD5-10_IM3R7-10.SPD9_DSM-H6.IonXpress_009.fastq -f 0 -o r440SPD9_DSM-H6-matches-longreads-top10.f0.eval5e-6 -t temp1

Total time = 2376.49s
Reported 48523340 pairwise alignments, 48523340 HSPs.
555852 queries aligned.
real	39m37.165s

# move diamond alignments 
cd ~/Elixir/metagenomics/SPlab/run440$ for i in  ../../nr/r440*
> do
> mv $i .
> done

mv ../../nr/r431SPD3matches-longreads-top10.f0.eval5e-6 .
mv ../../nr/r431SPD4matches-longreads-top10.f0.eval5e-6 .