##FastQC	0.11.8
>>Basic Statistics	pass
#Measure	Value
Filename	SPD3.s10q25.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4585844
Sequences flagged as poor quality	0
Sequence length	2-256
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.942915633414483	27.0	26.0	28.0	24.0	29.0
2	26.766159729812003	27.0	26.0	28.0	23.0	29.0
3	26.73558958645027	27.0	26.0	28.0	23.0	29.0
4	26.779657110108747	27.0	26.0	28.0	23.0	29.0
5	26.764693730189375	27.0	26.0	28.0	23.0	29.0
6	26.810166510864853	27.0	26.0	28.0	23.0	29.0
7	26.87203243559384	27.0	26.0	28.0	23.0	30.0
8	26.887833734030682	27.0	26.0	28.0	23.0	30.0
9	26.93873422141491	27.0	26.0	28.0	23.0	30.0
10-14	27.077610891577706	27.0	26.0	28.8	23.0	30.0
15-19	27.05392508633374	27.0	26.0	28.4	23.0	30.0
20-24	26.970929980521085	27.0	26.0	28.0	23.0	29.8
25-29	26.88263590264731	27.0	26.0	28.0	23.0	29.0
30-34	26.858835498853885	27.0	26.0	28.0	23.0	29.0
35-39	26.85525424626046	27.0	26.0	28.0	23.0	29.0
40-44	26.837250105677846	27.0	26.0	28.0	23.0	29.0
45-49	26.7990953337132	27.0	26.0	28.0	23.0	29.0
50-54	26.786861257807765	27.0	26.0	28.0	23.0	29.0
55-59	26.78463163886844	27.0	26.0	28.0	23.0	29.0
60-64	26.78145940003421	27.0	26.0	28.0	23.0	29.0
65-69	26.775086299897065	27.0	26.0	28.0	23.0	29.0
70-74	26.769626519723023	27.0	26.0	28.0	23.0	29.0
75-79	26.777620046843555	27.0	26.0	28.0	23.0	29.0
80-84	26.78749228860695	27.0	26.0	28.0	23.0	29.0
85-89	26.793507284861203	27.0	26.0	28.0	23.0	29.0
90-94	26.805533408223646	27.0	26.0	28.0	23.0	29.0
95-99	26.808495376200653	27.0	26.0	28.0	23.0	29.0
100-104	26.818385295141088	27.0	26.0	28.0	23.0	29.0
105-109	26.82998895914818	27.0	26.0	28.0	23.0	29.0
110-114	26.839665742754768	27.0	26.0	28.0	23.0	29.0
115-119	26.848255705193274	27.0	26.0	28.0	23.0	29.0
120-124	26.865083442163552	27.0	26.0	28.0	23.0	29.0
125-129	26.865817112966774	27.0	26.0	28.0	23.0	29.0
130-134	26.876206449798623	27.0	26.0	28.0	23.0	29.0
135-139	26.85994555447659	27.0	26.0	28.0	23.0	29.0
140-144	26.868065205922683	27.0	26.0	28.0	23.0	29.0
145-149	26.858504437705655	27.0	26.0	28.0	23.0	29.0
150-154	26.870211151718998	27.0	26.0	28.0	23.0	29.0
155-159	26.863241607728234	27.0	26.0	28.0	23.0	29.0
160-164	26.91459345986384	27.2	26.0	28.0	23.0	29.0
165-169	26.86736593747863	27.0	26.0	28.0	23.0	29.0
170-174	26.824303058638446	27.0	26.0	28.0	23.0	29.0
175-179	26.84776468062961	27.0	26.0	28.0	23.0	29.0
180-184	26.860915419616624	27.2	25.8	28.0	23.4	29.0
185-189	26.779982929604746	27.0	26.0	28.0	23.0	29.0
190-194	26.926516861073264	27.4	26.0	28.0	23.2	29.2
195-199	26.96555416234039	27.4	26.0	28.0	23.2	29.0
200-204	26.84888165353314	27.2	25.8	28.0	23.0	29.0
205-209	26.7538583627629	27.0	25.0	28.0	23.0	29.0
210-214	26.875409330780364	NaN	NaN	NaN	NaN	NaN
215-219	26.43900010355183	NaN	NaN	NaN	NaN	NaN
220-224	26.904552429667525	NaN	NaN	NaN	NaN	NaN
225-229	26.498207282913164	NaN	NaN	NaN	NaN	NaN
230-234	26.768701298701302	NaN	NaN	NaN	NaN	NaN
235-239	26.779285714285713	NaN	NaN	NaN	NaN	NaN
240-244	26.262380952380955	NaN	NaN	NaN	NaN	NaN
245-249	26.166666666666664	NaN	NaN	NaN	NaN	NaN
250-254	26.0	NaN	NaN	NaN	NaN	NaN
255-256	26.5	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
23	1.0
24	3595.0
25	591678.0
26	2250144.0
27	1609861.0
28	121447.0
29	8054.0
30	867.0
31	149.0
32	30.0
33	12.0
34	4.0
35	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.093751989819104	27.48876760744587	19.060460844285153	31.35701955844987
2	17.93543347745802	25.846452692241602	35.329614352341686	20.888499477958693
3	16.79254347029209	29.86643866010821	31.095746399836194	22.24527146976351
4	18.66956907823863	31.071555988600867	30.7600817513562	19.498793181804306
5	19.74631492376795	32.42360979312963	28.475446277620346	19.354629005482078
6	19.295899662029914	30.293926658109044	30.620247124915608	19.78992655494544
7	20.530574372978545	30.605023207544267	29.28040122337069	19.5840011961065
8	18.874646979999536	29.277926173218706	31.420114710367887	20.42731213641387
9	19.59835054194928	28.27587127098911	31.460807053029793	20.66497113403181
10-14	20.304986415399274	29.620692973707275	29.29205380392681	20.782266806966643
15-19	20.3638210349671	29.609010526150144	29.268618219909538	20.75855021897322
20-24	20.422423797758128	29.61953694137734	29.22691236914726	20.73112689171727
25-29	20.453839146525358	29.706293078499947	29.19370048613354	20.64616728884116
30-34	20.56536055871481	29.61101855958242	29.0457601347886	20.777860746914172
35-39	20.63810249902386	29.671648192719115	29.01843203298473	20.671817275272296
40-44	20.724826273624576	29.50765281281813	28.910453090190174	20.85706782336712
45-49	20.700083724253414	29.416040573849067	29.01027449693034	20.87360120496718
50-54	20.79750492917553	29.226654516681368	29.018186063404762	20.95765449073834
55-59	20.8796099912324	29.191823346984613	29.000362823827935	20.928203837955053
60-64	20.92461216403381	29.068420552096242	28.980958903516	21.026008380353947
65-69	20.92871020317802	29.041381006364002	29.087075749123866	20.94283304133411
70-74	20.958537913308774	28.868004427592997	29.04088983501167	21.132567824086564
75-79	20.9856961383113	28.86709639866754	29.10314747883131	21.044059984189847
80-84	21.0490348856289	28.678754584660794	29.090627137018636	21.181583392691667
85-89	20.919891697745772	28.77059947456413	29.16852299241241	21.14098583527769
90-94	21.01996409992026	28.650928581444557	29.075785724102378	21.253321594532807
95-99	21.00867251194483	28.698935805012017	29.241723803366277	21.050667879676883
100-104	21.038739048994987	28.65246990836014	29.021108245907286	21.287682796737588
105-109	20.977243319415994	28.76436043983676	29.111813671819746	21.146582568927492
110-114	21.057084482650147	28.565586391647436	29.21411565245322	21.163213473249204
115-119	21.05767372108189	28.68243236066946	29.13488356285142	21.125010355397233
120-124	21.149239388409015	28.54571285354661	29.058326017541287	21.246721740503087
125-129	21.052380340389593	28.658453742633156	29.149393210567865	21.139772706409385
130-134	21.239270042006645	28.56550519036266	29.03542675492504	21.159798012705654
135-139	21.107737764465107	28.66998278689635	29.124029221615324	21.098250227023218
140-144	21.27751932132376	28.5106302667711	28.97773919279803	21.234111219107113
145-149	20.921142765040095	28.81223892224416	28.964016601523152	21.302601711192594
150-154	21.083838675522188	28.258735200693042	29.19434016748484	21.46308595629993
155-159	20.991703105729343	28.697163359120974	28.74761744590201	21.563516089247674
160-164	21.162684774957558	28.429464618442303	28.802119995031262	21.60573061156888
165-169	21.342618044102377	28.477185266630016	28.464968541933906	21.7152281473337
170-174	21.531922589980105	28.27816965093145	28.838849701573523	21.351058057514923
175-179	21.380697050938338	28.364611260053618	28.203753351206434	22.05093833780161
180-184	21.607730263157894	28.351151315789476	28.824013157894733	21.217105263157894
185-189	21.92723697148476	29.006882989183875	28.38413634873812	20.681743690593247
190-194	21.610169491525426	28.177966101694917	28.49576271186441	21.716101694915256
195-199	20.859444941808416	27.84243509400179	29.45389435989257	21.844225604297225
200-204	25.547445255474454	26.423357664233578	29.927007299270077	18.1021897810219
205-209	21.99546485260771	28.798185941043087	28.117913832199548	21.08843537414966
210-214	17.857142857142858	27.77777777777778	29.761904761904763	24.6031746031746
215-219	20.125786163522015	26.41509433962264	30.18867924528302	23.270440251572328
220-224	18.085106382978726	28.723404255319153	28.723404255319153	24.46808510638298
225-229	29.72972972972973	29.72972972972973	28.37837837837838	12.162162162162163
230-234	14.035087719298245	17.543859649122805	36.84210526315789	31.57894736842105
235-239	14.285714285714285	35.714285714285715	21.428571428571427	28.57142857142857
240-244	11.11111111111111	22.22222222222222	55.55555555555556	11.11111111111111
245-249	21.428571428571427	21.428571428571427	42.857142857142854	14.285714285714285
250-254	0.0	60.0	20.0	20.0
255-256	50.0	50.0	0.0	0.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7316.0
1	7318.0
2	7467.5
3	8384.5
4	11091.5
5	17299.5
6	25479.5
7	31816.0
8	36708.5
9	40329.0
10	43487.0
11	47325.0
12	51515.0
13	58315.5
14	68328.0
15	83090.0
16	95169.0
17	106841.0
18	121939.0
19	140195.5
20	156477.0
21	175921.0
22	197263.5
23	219575.0
24	243504.0
25	274959.5
26	309822.0
27	333842.0
28	367096.0
29	403510.0
30	435092.5
31	469277.8333333334
32	506159.1666666667
33	535795.3333333333
34	561474.3333333333
35	590245.5
36	615175.5
37	632257.6666666666
38	645754.6666666667
39	656469.5000000001
40	661564.0
41	660617.9999999999
42	654382.0000000001
43	643653.5000000001
44	626762.166666667
45	602598.9999999999
46	572923.3333333333
47	545120.5
48	519892.83333333326
49	499067.49999999994
50	450931.3333333333
51	403336.1666666666
52	384797.49999999994
53	359479.0
54	328511.6666666666
55	294043.8333333333
56	263493.49999999994
57	241861.83333333334
58	219517.83333333334
59	194107.33333333334
60	177138.5
61	159744.8333333333
62	143869.3333333333
63	130491.0
64	113925.5
65	97217.5
66	83760.0
67	78880.5
68	71277.5
69	59951.5
70	52457.0
71	47330.5
72	41281.5
73	36166.0
74	33794.5
75	27806.0
76	22284.0
77	20156.5
78	17928.0
79	15128.0
80	12631.0
81	10917.0
82	9038.5
83	7780.0
84	6723.0
85	5788.0
86	4549.5
87	4150.5
88	3750.0
89	3318.5
90	3008.0
91	2725.0
92	2387.5
93	1771.0
94	1159.0
95	635.0
96	370.5
97	309.0
98	297.0
99	296.0
100	296.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0
20-24	0.0
25-29	0.0
30-34	0.0
35-39	0.0
40-44	0.0
45-49	0.0
50-54	0.0
55-59	0.0
60-64	0.0
65-69	0.0
70-74	0.0
75-79	0.0
80-84	0.0
85-89	0.0
90-94	0.0
95-99	0.0
100-104	0.0
105-109	0.0
110-114	0.0
115-119	0.0
120-124	0.0
125-129	0.0
130-134	0.0
135-139	0.0
140-144	0.0
145-149	0.0
150-154	0.0
155-159	0.0
160-164	0.0
165-169	0.0
170-174	0.0
175-179	0.0
180-184	0.0
185-189	0.0
190-194	0.0
195-199	0.0
200-204	0.0
205-209	0.0
210-214	0.0
215-219	0.0
220-224	0.0
225-229	0.0
230-234	0.0
235-239	0.0
240-244	0.0
245-249	0.0
250-254	0.0
255-256	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
0-9	259052.0
10-19	1145077.0
20-29	777714.0
30-39	605407.0
40-49	482881.0
50-59	376130.0
60-69	282577.0
70-79	202794.0
80-89	144665.0
90-99	102441.0
100-109	74344.0
110-119	52353.0
120-129	34716.0
130-139	21466.0
140-149	12276.0
150-159	6311.0
160-169	3076.0
170-179	1412.0
180-189	708.0
190-199	279.0
200-209	105.0
210-219	37.0
220-229	9.0
230-239	7.0
240-249	6.0
250-257	1.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	94.33189740345462
#Duplication Level	Percentage of deduplicated	Percentage of total
1	96.87309686351915	91.38223034484409
2	2.1399776647513793	4.03736307034023
3	0.48064903450617424	1.3602160623031785
4	0.19140614662780397	0.7222281994433838
5	0.09939207953269186	0.4687921724596945
6	0.05341127639182508	0.30230322268687204
7	0.04112625144676565	0.27156621324454816
8	0.025681229937783878	0.19380473181484448
9	0.019488227519421603	0.16545253310435334
>10	0.07548697407528032	1.0794650447842715
>50	2.842516917787188E-4	0.016578404974623043
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.1806236758162728E-5	0.0
2	0.0	0.0	0.0	2.1806236758162728E-5	0.0
3	0.0	0.0	0.0	2.1806236758162728E-5	0.0
4	0.0	0.0	0.0	2.1806236758162728E-5	0.0
5	0.0	4.3612473516325456E-5	0.0	2.1806236758162728E-5	0.0
6	0.0	4.3612473516325456E-5	0.0	2.1806236758162728E-5	0.0
7	0.0	4.3612473516325456E-5	0.0	2.1806236758162728E-5	0.0
8	0.0	4.3612473516325456E-5	0.0	2.1806236758162728E-5	0.0
9	0.0	4.3612473516325456E-5	0.0	2.1806236758162728E-5	0.0
10-14	0.0	4.3612473516325456E-5	0.0	2.1806236758162728E-5	0.0
15-19	0.0	4.3612473516325456E-5	0.0	2.1806236758162728E-5	0.0
20-24	1.7444989406530183E-5	4.3612473516325456E-5	0.0	3.4889978813060366E-5	1.3083742054897636E-5
25-29	7.414120497775328E-5	4.3612473516325456E-5	0.0	4.3612473516325456E-5	3.925122616469291E-5
30-34	1.0030868908754854E-4	4.3612473516325456E-5	0.0	4.3612473516325456E-5	4.3612473516325456E-5
35-39	1.0903118379081364E-4	4.3612473516325456E-5	0.0	4.3612473516325456E-5	4.3612473516325456E-5
40-44	1.0903118379081364E-4	4.3612473516325456E-5	2.1806236758162728E-5	4.3612473516325456E-5	4.3612473516325456E-5
45-49	1.0903118379081364E-4	4.3612473516325456E-5	2.1806236758162728E-5	5.2334968219590546E-5	4.3612473516325456E-5
50-54	1.0903118379081364E-4	4.3612473516325456E-5	2.1806236758162728E-5	6.541871027448818E-5	4.3612473516325456E-5
55-59	1.0903118379081364E-4	5.2334968219590546E-5	2.1806236758162728E-5	6.541871027448818E-5	4.3612473516325456E-5
60-64	1.0903118379081364E-4	6.541871027448818E-5	2.6167484109795273E-5	6.541871027448818E-5	4.3612473516325456E-5
65-69	1.0903118379081364E-4	6.541871027448818E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
70-74	1.0903118379081364E-4	6.541871027448818E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
75-79	1.0903118379081364E-4	7.850245232938582E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
80-84	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
85-89	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
90-94	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
95-99	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
100-104	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
105-109	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
110-114	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
115-119	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
120-124	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
125-129	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
130-134	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
135-139	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
140-144	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
145-149	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
150-154	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
155-159	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
160-164	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
165-169	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
170-174	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
175-179	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
180-184	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
185-189	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
190-194	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
195-199	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
200-204	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
205-209	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
210-214	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
215-219	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
220-224	1.3083742054897636E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
225-229	1.526436573071391E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
230-234	1.526436573071391E-4	8.722494703265091E-5	4.3612473516325456E-5	6.541871027448818E-5	4.3612473516325456E-5
235-239	1.700886467136693E-4	1.0466993643918109E-4	6.105746292285564E-5	8.286369968101837E-5	4.3612473516325456E-5
240-244	1.7444989406530182E-4	1.0903118379081364E-4	6.541871027448818E-5	8.722494703265091E-5	4.3612473516325456E-5
245	1.9625613082346457E-4	1.3083742054897636E-4	0.0	1.0903118379081364E-4	6.541871027448818E-5
>>END_MODULE
