FastQCFastQC Report
Mon 14 Oct 2019
SPD2.s10q25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSPD2.s10q25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences540162
Sequences flagged as poor quality0
Sequence length3-243
%GC58

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGCATTC7643914.151125032860513No Hit
GGCAGCATTCCGAGTA241094.463290642436898No Hit
GGCAGCATTCCGAG223884.1446825211695755No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTG152742.827670217453283No Hit
GGCAGCAT152362.8206352908942134No Hit
GGCAGCATTCCGAGTAACTCCGCA127642.362994805262125No Hit
GGCAGCATTCCGAGTAACTCCTCA123602.2882024281604405No Hit
GGCAGC113902.108626671257882No Hit
GGCAGCATTCCGAGTAACTCCT112942.090854225213917No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTC110732.0499405733835405No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGT110132.0388327946060625No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCG104261.9301616922330709No Hit
GGCAGCATTCCGAGTAACTCCTCAAC101491.878880780210381No Hit
GGCAGCATTCCGAGTAACTCCGCAACC99341.8390779062577522No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGA96061.7783553822742066No Hit
GGCAGCATTCCGA94341.7465130831121036No Hit
GGCAGCATTCCGAGTAACTC93431.7296662852995952No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTC84321.561013177528223No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAG76661.4192038684690889No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGT69051.2783202076414113No Hit
GGCAGCATTCCGAGTAAC66471.2305567588982564No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAG65951.2209300172911088No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGA65501.2125991832080008No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGA63981.1844594769717232No Hit
GGCAGCATTCCGAGTAACTCCG52470.9713752540904395No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGA51700.9571202713260096No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAG48110.8906587283074338No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGG47980.8882520429056469No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCC46980.869739078276517No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGG43420.8038329241968151No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGC43160.7990195533932413No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGC42740.7912441082490068No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCG41810.774027051143916No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTC40090.7421847519818128No Hit
GGCAGCATTCCG37610.696272599701571No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGA32570.6029672579707569No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAG32470.601115961507844No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCAC29520.5465027158519111No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCG29390.5440960304501242No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGA27890.5163265835064296No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGC24450.4526419851822231No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCT21840.4043231475001944No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAG21820.40395288820761177No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGC21790.40339749926873786No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGC20980.38840199791914276No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGC20980.38840199791914276No Hit
GGCAGCATTCCGAGT20910.3871060903951037No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAAGCAG20290.37562805232504326No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAG19450.36007716203657425No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCT18610.34452627174810524No Hit
GGCAGCATTCCGAGTAACACCGCA18370.34008316023711405No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAG18320.3391575120056576No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGC17640.32656869605784933No Hit
GGCAGCATTCCGAGTAACT16600.30731521284355434No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCGCG16010.2963925637123678No Hit
GGCAGCATTCCGAGTAACACCGCAACC15800.29250484114025055No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATCT15790.29231971149395924No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCT15720.2910238039699201No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGG15340.2839888774108508No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGC14170.262328708794769No Hit
GGCAGCATTCCGAGTAACTCCTC13890.25714507869861264No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCG13860.2565896897597387No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGC13650.2527019671876215No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGA13300.24622242956742604No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAG13240.2451116516896783No Hit
GGCAGCATTCCGAGTAACACCGCAACCCG13210.2445562627508044No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAGCTGCTGAATCTTC13080.24214957734901751No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAGCTGCT12650.2341890025584917No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCG12590.2330782246807439No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGA12430.23011615034008318No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAG12160.2251176498902181No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGA12100.22400687201247033No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGA11750.2175273343922749No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTC11150.20641955561479705No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAG11020.20401287021301018No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAG10610.19642255471506698No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAGGG10550.19531177683731918No Hit
GGCAGCATTCCGAGTAACTCCTCAACCT10420.1929050914355323No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGT10230.18938762815599763No Hit
GGCAGCATTCCGAGTAACTCCGC9890.1830932201820935No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTG9730.18013114584143278No Hit
GGCAGCATTCCGAGTAACTCCTCAACC9440.17476238609898512No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTA9440.17476238609898512No Hit
GGCAGCATTCCGAGTAACAC9360.17328134892865474No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGC9170.1697638856491201No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGC9040.1673572002473332No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAG8650.1601371440419726No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTC8560.15847097722535092No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGA8220.1521765692514468No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGC8180.1514360506662816No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAAT8080.14958475420336861No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGA8080.14958475420336861No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCTGAAGAAGCAG8050.14902936526449473No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAG8000.14810371703303823No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCT7850.14532677233866875No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAGGGGC7700.1425498276442993No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAGCTGCTGAATCT7390.13681080860926909No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGT7260.1344041232074822No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGT7180.13292308603715183No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGG7050.13051640063536496No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGT7050.13051640063536496No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATCTTC7050.13051640063536496No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGCCGAATCT6980.12922049311132586No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAG6820.1262584187706651No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAG6730.12459225195404341No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGA6640.12292608513742175No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAGGGGCCGCG6620.12255582584483915No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGGGC6600.12218556655225654No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTA6550.12125991832080006No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAG6350.11755732539497411No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAG6170.11422499176173073No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGA6070.11237369529881777No Hit
GGCAGCATTCCGAGTAACTCCTCAGCCCGGAGTTC5980.11070752848219609No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTG5970.11052239883590478No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGC5940.10996700989703088No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTG5800.1073751948489527No Hit
GGCAGCATTCCGAGTAACACCG5730.10607928732491363No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGC5730.10607928732491363No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGA5640.10441312050829196No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGCCGA5640.10441312050829196No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCG5590.10348747227683545No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCTGA5410.1001551386435921No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)