FastQCFastQC Report
Mon 14 Oct 2019
SPD2.s10q20l20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSPD2.s10q20l20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences948249
Sequences flagged as poor quality0
Sequence length20-251
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAG12999213.70863560098666No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAG880429.284692100914423No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAG629406.637497113100039No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAG293443.094545841862211No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAG283052.9849754653049994No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAG223532.3572922302053576No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAAGCAG210222.2169282540767243No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCTGAAGAAGCAG174771.8430813003757454No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATCTTC134471.4180874432770294No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATCT121411.2803599054678676No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCT106991.1282901432007837No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCG96921.0220944076924943No Hit
GGCAGCATTCCGAGTAACTCCT90660.9560779921729419No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCG79600.8394419609195476No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGC70120.7394682198452095No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAAT68830.7258641981167393No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGG62380.6578440894743891No Hit
GGCAGCATTCCGAGTAACTCCGCAACC57840.6099663695927968No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGA56640.5973114656593362No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCT54800.5779072796280302No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGC54150.5710525399974058No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGC53540.5646196304978967No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTG52470.553335674490561No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGG51500.5431062938110137No Hit
GGCAGCATTCCGAGTAACTC51090.5387825349670815No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAGCTGCTGAATCTTC50400.5315059652053417No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGA48460.5110472038462471No Hit
GGCAGCATTCCGAGTAACTCCTCA45550.4803590618076054No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCC45500.4798317741437112No Hit
GGCAGCATTCCGAGTAACTCCTCAGCCCGGAGTTCCACCCGAGGAAGCGG44010.4641186017596644No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGA43690.4607439607107416No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTC42840.4517800704245404No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAGCTGCT40980.4321649693276766No Hit
GGCAGCATTCCGAGTAACTCCG39570.4172954572058605No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGT39400.4155026791486202No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGT39320.41465901888638956No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGA37520.39567666298619875No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGC36650.3865018576344399No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCG36590.3858691124377669No Hit
GGCAGCATTCCGAGTAACTCCGCA35450.37384695370097937No Hit
GGCAGCATTCCGAGTAACTCCTCAAC35170.3708941427831719No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAG34540.36425031821810516No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCT32850.3464279951784816No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGA30870.32554740368827173No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTC30710.3238600831638103No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGA30320.3197472393854357No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGC29250.3084632833781001No Hit
GGCAGCATTCCGAGTAACTCCTCAACC29000.3058268450586291No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGC28870.3044558971325042No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGC28510.3006594259524661No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATC27900.29422651645295694No Hit
GGCAGCATTCCGAGTAACTCCTCAACCAGGAGTTCCGCCCGAAGAAGCAG26920.2838916782406309No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCGCG26040.2746114153560932No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCCGAGGAAGCAG26040.2746114153560932No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAGCTGCTGAATCT22600.23833402408017304No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATCTTC22570.23801765148183654No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGC22450.23675216108849048No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTC22350.2356975857607021No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAA22300.23517029809680792No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCCGAGGAAGCAG22200.2341157227690195No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTG21220.22378088455669345No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAAGAAGCAG20680.21808617778663622No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAA20590.21713705999162666No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGG20470.21587156959828063No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCC20310.2141842490738192No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCT19840.20922774503321384No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATCT19440.20500944372206037No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCG18540.195518265771965No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCT17520.18476159742852352No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCT17490.184445224830187No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGA17410.18360156456795632No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAG17240.18180878651071608No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGT17170.1810705837812642No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGT16410.17305581129007255No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAGCTGCTGATCTTC16380.17273943869173602No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGCC16310.17200123596228417No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAG15830.16693927438889997No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTG15570.16419737853665017No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAG15450.16293188814330414No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGCCGAATCT15240.16071727995494856No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAGGGGC15180.16008453475827553No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCG15100.15924087449604482No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCAC13920.14679688562814197No Hit
GGCAGCATTCCGAGTAACTCCTCAACCGGGAGTTCCACCCGAGGAAGCAG13910.14669142809536315No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTG13740.1448986500381229No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGC13660.1440549897758922No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAG13650.14394953224311335No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCC13640.1438440747103345No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAAGCAGGAGCTGCGGTAGCTGCTGAATCT13600.14342224457921918No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAGGG13580.1432113295136615No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTA13550.14289495691532497No Hit
GGCAGCATTCCGAGTAACTCC13300.14025851859585406No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGG13130.1384657405386138No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAG12530.13213828857188353No Hit
GGCAGCATTCCGAGTAACTCCTC12400.13076734064575865No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGC12280.1295018502524126No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAGGGGCCGCG12220.12886910505573956No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAAGCAGGAGCTGCGGTAGCTGCT12140.12802544479350889No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGG12040.1269708694657205No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGA12020.12675995440016283No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCG11970.12623266673626865No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGC11920.12570537907237445No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGGGC11720.12359622841679771No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGC11710.12349077088401886No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGGCTGAAGAAGCAG11490.12117070516288442No Hit
GGCAGCATTCCGAGTAACACCGCAACC11390.12011612983509605No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCT11350.11969429970398071No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAAT11180.11790152164674046No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCTGAAGAAGCAGGGGC11130.11737423398284628No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTA11070.11674148878617326No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGC10470.110414036819443No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAA10470.110414036819443No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGG10430.10999220668832764No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCGG10420.1098867491555488No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAGGGGCCGCGGTAGCTGC10380.10946491902443346No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAG10330.10893763136053927No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAAG10320.10883217382776043No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAG10290.1085158012294239No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAG10090.10640665057384717No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGC10070.10619573550828948No Hit
GGCAGCATTCCGAGTAACTCCTCACCTG10050.10598482044273182No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCCCCTGAAGAAGCAG10040.10587936290995298No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGCCGAATCTTC9660.10187197666435716No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGG9650.10176651913157832No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAG9640.10166106159879948No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGA9600.10123923146768413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)