FastQCFastQC Report
Mon 14 Oct 2019
SPD2.s10q20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSPD2.s10q20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1028775
Sequences flagged as poor quality0
Sequence length4-251
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAG12999212.635610313236617No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAG880428.557945128915458No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAG629406.1179558212437115No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAG293442.8523243663580473No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAG283052.7513304658453013No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAG223532.172778304293942No Hit
GGCAGCATTC216012.099681660226969No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAAGCAG210222.043401132414765No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCTGAAGAAGCAG174771.6988165536681976No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATCTTC134471.307088527617798No Hit
GGCAGCATTCCGAG129471.2584870355519915No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATCT121411.1801414303419113No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCT106991.0399747272241258No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCG96920.9420913222035917No Hit
GGCAGCATTCCGAGTAACTCCT90660.881242254137202No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCG79600.7737357536876383No Hit
GGCAGCATTCCGAGTA72660.7062768827002989No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGC70120.6815873247308692No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAAT68830.6690481397778912No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGG62380.606352215013001No Hit
GGCAGCATTCCGAGTAACTCCGCAACC57840.5622220602172487No Hit
GGCAGCATTCCG56980.55386260358193No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGA56640.5505577021214552No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCT54800.5326723530412384No Hit
GGCAGC54650.5312143082792642No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGC54150.5263541590726836No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGC53540.5204247770406552No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTG52470.5100240577385726No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGG51500.5005953682778062No Hit
GGCAGCATTCCGAGTAACTC51090.49661004592840996No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAGCTGCTGAATCTTC50400.4899030400233287No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGA48460.47104566110179585No Hit
GGCAGCATTCCGAGTAACTCCTCA45550.4427595927194965No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCC45500.4422735777988384No Hit
GGCAGCATTCCGA44120.4288595659886758No Hit
GGCAGCATTCCGAGTAACTCCTCAGCCCGGAGTTCCACCCGAGGAAGCGG44010.4277903331632281No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGA43690.4246798376710165No Hit
GGCAGCAT43220.4201112974168307No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTC42840.41641758401982937No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAGCTGCT40980.39833782897134945No Hit
GGCAGCATTCCGAGTAAC39960.3884231245899249No Hit
GGCAGCATTCCGAGTAACTCCG39570.384632208208792No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGT39400.38297975747855456No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGT39320.3822021336055017No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGA37520.3647055964618114No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGC36650.35624893684236103No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCG36590.3556657189375714No Hit
GGCAGCATTCCGAGTAACTCCGCA35450.3445845787465675No Hit
GGCAGCATTCCGAGTAACTCCTCAAC35170.34186289519088237No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAG34540.33573910719059075No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCT32850.31931180287234817No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGA30870.30006561201428883No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTC30710.298510364268183No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGA30320.2947194478870501No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGC29250.28431872858496754No Hit
GGCAGCATTCCGAGTAACTCCTCAACC29000.28188865398167723No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGC28870.2806250151879663No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGC28510.2771257077592282No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATC27900.2711963257271998No Hit
GGCAGCATTCCGAGTAACTCCTCAACCAGGAGTTCCGCCCGAAGAAGCAG26920.2616704332823018No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCGCG26040.25311657067871984No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCCGAGGAAGCAG26040.25311657067871984No Hit
GGCAGCATTCC24990.24291025734490046No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAGCTGCTGAATCT22600.21967874413744504No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATCTTC22570.21938713518505018No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGC22450.2182206993754708No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTC22350.2172486695341547No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAA22300.21676265461349664No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCCGAGGAAGCAG22200.2157906247721805No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTG21220.20626473232728246No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAAGAAGCAG20680.20101577118417532No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAA20590.2001409443269908No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGG20470.1989745085174115No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCC20310.19741926077130567No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCT19840.19285072051711988No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAATCT19440.18896260115185537No Hit
GGCAGCATTCCGAGTAACT19410.18867099219946054No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCG18540.1802143325800102No Hit
GGCAGCATTCCGAGT17830.17331292070666568No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCT17520.1702996281985857No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCT17490.17000801924619086No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGA17410.16923039537313794No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAG17240.16757794464290054No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGT17170.16689752375397923No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGT16410.15951009695997667No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAGCTGCTGATCTTC16380.15921848800758184No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGCC16310.15853806711866053No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAG15830.15387232388034314No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTG15570.15134504629292117No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAG15450.15017861048334183No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCGCGGTAGCTGCCGAATCT15240.14813734781657797No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAGGGGC15180.1475541299117883No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCG15100.1467765060387354No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCAC13920.13530655391120505No Hit
GGCAGCATTCCGAGTAACTCCTCAACCGGGAGTTCCACCCGAGGAAGCAG13910.13520935092707348No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTG13740.13355690019683603No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGC13660.13277927632378314No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAG13650.1326820733396515No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCC13640.1325848703555199No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAAGCAGGAGCTGCGGTAGCTGCTGAATCT13600.13219605841899346No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAGGG13580.13200165245073026No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTA13550.1317100434983354No Hit
GGCAGCATTCCGAGTAA13530.13151563753007217No Hit
GGCAGCATTCCGAGTAACTCC13300.1292799688950451No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGG13130.12762751816480766No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAG12530.1217953391169109No Hit
GGCAGCATTCCGAGTAACTCCTC12400.12053170032319993No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGC12280.11936526451362056No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAGGGGCCGCG12220.11878204660883088No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAAGCAGGAGCTGCGGTAGCTGCT12140.11800442273577798No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGG12040.11703239289446185No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGA12020.11683798692619864No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGGGGCCG11970.11635197200554057No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGC11920.11586595708488251No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAGGGGC11720.11392189740225024No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGC11710.11382469441811864No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGGCTGAAGAAGCAG11490.11168622876722314No Hit
GGCAGCATTCCGAGTAACACCGCAACC11390.11071419892590703No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCT11350.11032538698938056No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGTAGCTGCTGAAT11180.10867293625914315No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCTGAAGAAGCAGGGGC11130.10818692133848509No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTA11070.10760370343369542No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGC10470.10177152438579865No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAA10470.10177152438579865No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGG10430.1013827124492722No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCGG10420.10128550946514059No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAGGGGCCGCGGTAGCTGC10380.10089669752861413No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAG10330.10041068260795608No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAAG10320.10031347962382445No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAG10290.10002187067142962No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)