FastQCFastQC Report
Mon 14 Oct 2019
SPD1.s10q25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSPD1.s10q25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329959
Sequences flagged as poor quality0
Sequence length2-249
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCACCA18832514.16021095387151No Hit
ATGTCACCACAAACAGAGAC1050937.901972917962133No Hit
ATGTCACCACAA773155.813337102873096No Hit
ATGTCACCACAAACAGAGACTAA580714.366375204047643No Hit
ATGTCACCACAAACAGAGACTAAAGCA574904.322689646823699No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAA569914.285169693201069No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGAT568334.273289627725366No Hit
ATGTCACCACAAACAGAGACTAAAG557264.190053979107627No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGT520183.911248391867719No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGT519483.905985071720256No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTG422243.1748347129497976No Hit
ATGTCACCACAAACAGA387692.9150522685285787No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTA303972.285559178891981No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGT301282.265332991468158No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTG227891.7135114691505526No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAA224121.6851647306420723No Hit
ATGTCACCACAAACA170221.2798890792874065No Hit
ATGTCAC167951.2628208839520616No Hit
ATGTCACCACAAACAG165801.2466549720705675No Hit
ATGTCACCACAAAC164511.236955424941671No Hit
ATGTCACCACAAACAGAGA163111.2264287846467448No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAA149991.1277791270257203No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAG123160.9260435848022383No Hit
ATGTCACCACAAACAGAGACT117820.8858919711058763No Hit
ATGTCACCACAAACAGAGACTAAAGCAAG101720.7648356077142228No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGT95480.7179168681139795No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGC94960.7140069731472926No Hit
ATGTCACCACAAACAGAG94830.7130294994056208No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACT93210.7008486727786345No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAG89360.671900411967587No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAAT84470.6351323612231655No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAG75880.57054390398501No Hit
ATGTCACCACAAACAGAGACTAAAGC75550.5680626244869202No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTG66980.5036245478244066No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGT60760.45685618879980516No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGA60570.4554275733312079No Hit
ATGTCACCAC56390.42399803302207056No Hit
ATGTCACCACA56360.42377246215860787No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTC54670.4110653035168753No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGA52130.39196697041036604No Hit
ATGTCACCACAAACAGAGACTA49770.37422206248463297No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCA46140.3469279880056453No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTG44790.3367772991498234No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAA39510.29707682718038675No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTG34380.25850420952826364No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTAT31750.23872916383136625No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATA31530.23707497749930637No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGAT28320.2129388951087966No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGA27030.20323934797990012No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCGGGTGT19470.14639549038729766No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTAT18510.139177222756491No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAG17100.1285753921737437No Hit
ATGTC16290.12248497886025059No Hit
ATGTCACCACAAACAGAGACTAAAGCAGGTGTTGGATTCAAAGCTGGTGT14920.11218390942878691No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTA14640.11007858136980163No Hit
ATGTCACCACAAACAGAGACTAAAGCGAGTGTTGGATTCAAGGCCGGTGT14160.1064694475543983No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTA13780.10361221661720399No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGA13710.10308588460245767No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)