FastQCFastQC Report
Mon 14 Oct 2019
SPD1.s10q20l20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSPD1.s10q20l20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634464
Sequences flagged as poor quality0
Sequence length20-267
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGT45379827.764331303717917No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACT505893.0951431172543415No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAA501103.0658368737396477No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTA485042.96757836208078No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAG419122.5642657164672946No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAAT325971.9943541124185054No Hit
ATGTCACCACAAACAGAGACTAAAG304671.8640361610901188No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAA278391.7032495056483348No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTAT241571.4779768780468705No Hit
ATGTCACCACAAACAGAGACTAAAGCA224671.3745790668989957No Hit
ATGTCACCACAAACAGAGACTAA223101.3649734714254949No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAA213621.3069728057638468No Hit
ATGTCACCACAAACAGAGAC209191.2798691191730134No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGT186341.1400679366446738No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGA178831.0941201519274821No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTG178381.0913669557726569No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATA178141.0898985844900837No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGT172121.0530669381522018No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTG171961.0520880239638193No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTG165641.0134209135227206No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGAT162370.9934143547976584No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCGGGTGT147480.9023141531413357No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGT130490.7983657027624957No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAAAT117240.7172993715370911No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAG108980.6667629265618576No Hit
ATGTCACCACAAACAGAGACTAAAGCGAGTGTTGGATTCAAGGCCGGTGT106590.6521403958728977No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAA104460.6391086007400592No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGC98180.600686218846056No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGT96880.5927325410654503No Hit
ATGTCACCACAAACAGAGACTAAAGCAGGTGTTGGATTCAAAGCTGGTGT92680.5670360436204163No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGA90370.5529029700256476No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAG88310.5402994498502262No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTATTA85730.5245144585625624No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTAT84060.5142970417213227No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGG82320.5036513499226658No Hit
ATGTCACCACAAACAGAGACTAAAGCAA75380.46119094700158586No Hit
ATGTCACCACAAACAGAGACTAAAGCAAG73470.44950515887777276No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGAC69930.4278466824598156No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTA67570.41340769818117745No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATT67110.4105933198895784No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGAT51920.31765765413003894No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTC51590.31563864361650057No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCCGGTGT49520.30297394130430527No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGA48860.2989359202772285No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTG48080.294163713608865No Hit
ATGTCACCACAAACAGAGACTAAAGC44410.2717098694128473No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATT42420.2595346241948431No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTG41020.25096912504649843No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTCTAAATTGACT40300.2465640111987783No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAAATTGACT39810.24356608649685768No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGG39290.2403846153846154No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAA37830.23145202341562737No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTATAA36920.22588444896920337No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAAATTGA36200.22147933512148324No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTC36180.22135697084793549No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGGTGGATTCAAAGCTGGTGT35780.21890968537697986No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCA35580.21768604264150204No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTCTAA32830.2008609550286822No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAAATTG32670.19988204084029995No Hit
ATGTCACCACAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTG32500.1988419445151438No Hit
ATGTCACCACAAACAGAGACTAAAAGCAAGTGTTGGATTCAAAGCTGGTG32380.19810775887385712No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGT31320.19162245237582473No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGCTGGATTCAAAGCTGGTGT30890.18899162049454743No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCT30030.18372995673199288No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTCTAAATTGACTTAT29690.1816497640816806No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTA29500.1804873034829767No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGCT29210.17871302151653384No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTATTAT28470.17418554339526596No Hit
ATGTCACCACAAACAGAGACT28430.17394081484817042No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAGTGTTAAAG25520.1561368130469683No Hit
ATGTCACCACAAACAGAGACTAAAAGCA25090.15350598116569103No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAG24830.15191524560956987No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGTTCAA24730.15130342424183096No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTATAAATTGACT23810.14567466765863304No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTT23790.14555230338508526No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGA23150.14163664663155628No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTCTAAATTGACTTATTA22450.13735389705738396No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTCTAAAT22330.13661971141609724No Hit
ATGTCACCACAAACAGAGACTAAA21190.12964494782387376No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAA20090.12291491277874583No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTATAAAT19480.1191828024355385No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGGGT18960.11600133132329621No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTATAAATTGACTTAT17710.10835356422655991No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAGAG17330.10602864302915206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)