FastQCFastQC Report
Mon 14 Oct 2019
SPD1.s10q20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSPD1.s10q20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1810054
Sequences flagged as poor quality0
Sequence length4-267
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGT45379825.070964733648832No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACT505892.794888992262109No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAA501102.768425693377104No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTA485042.679699058702116No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAG419122.315511028952727No Hit
ATGTCACCACAA365742.0206027002509317No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAAT325971.8008854984436928No Hit
ATGTCACCACAAACAGAGACTAAAG304671.6832094512097429No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAA278391.5380204126506722No Hit
ATGTCACCA253621.4011736666419896No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTAT241571.3346010671504827No Hit
ATGTCACCACAAACAGAGACTAAAGCA224671.241233686950776No Hit
ATGTCACCACAAACAGAGACTAA223101.2325599125771938No Hit
ATGTCACCACAAACAGA218121.2050469212520731No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAA213621.1801857845125063No Hit
ATGTCACCACAAACAGAGAC209191.155711376566666No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGT186341.0294720489001985No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGA178830.9879815740303881No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTG178380.9854954603564314No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATA178140.9841695330636545No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGT172120.9509108568031672No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTG171960.9500269052746493No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTG165640.9151108198981909No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGAT162370.8970450605341056No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCGGGTGT147480.8147823214114055No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGT130490.7209177184769073No Hit
ATGTCACCACAAACA126030.69627756961947No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAAAT117240.6477154825215159No Hit
ATGTCAC109170.6031311773018927No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAG108980.6020814848617776No Hit
ATGTCACCACAAACAGAGACTAAAGCGAGTGTTGGATTCAAGGCCGGTGT106590.588877458904541No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAA104460.577109854181146No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGC98180.5424147566868172No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGT96880.5352326505176088No Hit
ATGTCACCACAAACAGAGACTAAAGCAGGTGTTGGATTCAAAGCTGGTGT92680.5120289228940131No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGA90370.4992668727010354No Hit
ATGTCACCACAAACAG90360.4992116257305031No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAG88310.48788599677136707No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTATTA85730.4736322783740154No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTAT84060.4644060342951094No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGG82320.4547930614224769No Hit
ATGTCACCACAAACAGAGACTAAAGCAA75380.41645166387301147No Hit
ATGTCACCACAAACAGAGACTAAAGCAAG73470.40589949250132873No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGAC69930.3863420649328694No Hit
ATGTCACCACAAAC68730.3797124284689849No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTA67570.3733037798872299No Hit
ATGTCACCAC67280.3717016177417911No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATT67110.37076241924274084No Hit
ATGTCACCACAAACAGAGA52690.2910962877350621No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGAT51920.2868422710040695No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTC51590.28501912097650123No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCCGGTGT49520.2735829980763005No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGA48860.269936698021164No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTG48080.2656274343196391No Hit
ATGTCACCACAAACAGAG47750.26380428429207087No Hit
ATGTCACCACAAACAGAGACTAAAGC44410.24535179613425898No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATT42420.23435764899831715No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTG41020.22662307312378527No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTCTAAATTGACT40300.22264529124545454No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAAATTGACT39810.21993818968936837No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGG39290.2170653472216851No Hit
ATGTCACCACA38530.2128665774612249No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAA37830.20899928952395896No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTATAA36920.20397181520551322No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAAATTGA36200.1999940333271825No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTC36180.19988353938611778No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGGTGGATTCAAAGCTGGTGT35780.19767366056482294No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCA35580.19656872115417554No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTCTAA32830.18137580425777353No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAAATTG32670.1804918527292556No Hit
ATGTCACCACAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTG32500.17955265423020528No Hit
ATGTCACCACAAACAGAGACTAAAAGCAAGTGTTGGATTCAAAGCTGGTG32380.17888969058381685No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGT31320.17303351170738554No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGCTGGATTCAAAGCTGGTGT30890.17065789197449358No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCT30030.1659066525087097No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTCTAAATTGACTTAT29690.16402825551060907No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTA29500.16297856307049402No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGCT29210.16137640092505529No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTATTAT28470.15728812510565984No Hit
ATGTCACCACAAACAGAGACT28430.15706713722353033No Hit
ATGTCACCACAAA26680.14739891738036545No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAGTGTTAAAG25520.14099026879861043No Hit
ATGTCACCACAAACAGAGACTAAAAGCA25090.1386146490657185No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAG24830.13717822783187683No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGTTCAA24730.13662575812655312No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTATAAATTGACT23810.131543036837575No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTT23790.13143254289651027No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGA23150.12789673678243854No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTCTAAATTGACTTATTA22450.12402944884517257No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTCTAAAT22330.12336648519878411No Hit
ATGTCACCACAAACAGAGACTAAA21190.11706833055809385No Hit
ATGTCACCACAACA20540.11347727747348973No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAA20090.11099116379953305No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTTAAAGATTATAAAT19480.10762109859705843No Hit
ATGTCACC19370.10701338192120236No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGGGT18960.10474825612937515No Hit
ATGTC18140.10021800454572073No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)