##FastQC	0.11.8
>>Basic Statistics	pass
#Measure	Value
Filename	SPD3.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	8160732
Sequences flagged as poor quality	0
Sequence length	25-391
%GC	40
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.14782472944829	27.0	23.0	28.0	20.0	29.0
2	24.64213296062167	26.0	23.0	28.0	19.0	29.0
3	24.388365161360525	26.0	23.0	28.0	17.0	29.0
4	24.260633604926618	26.0	23.0	28.0	16.0	29.0
5	24.099336554612993	26.0	22.0	28.0	15.0	29.0
6	24.108320430079065	26.0	22.0	28.0	15.0	29.0
7	24.171478612457804	26.0	22.0	28.0	15.0	29.0
8	24.201836550936854	26.0	22.0	28.0	15.0	29.0
9	24.30677627938278	26.0	22.0	28.0	16.0	29.0
10-19	24.749444363569346	26.0	23.0	28.0	18.7	29.0
20-29	24.79653237113618	26.0	23.0	28.0	19.4	29.0
30-39	24.605562751386255	26.0	23.0	28.0	19.0	29.0
40-49	24.378438218644582	26.0	22.5	28.0	18.2	29.0
50-59	24.116061910819717	25.5	22.0	27.2	17.3	28.3
60-69	23.89644593287867	25.0	22.0	27.0	16.5	28.0
70-79	23.709538144678557	25.0	21.9	27.0	16.0	28.0
80-89	23.58339224133619	25.0	21.0	27.0	15.8	28.0
90-99	23.50429085231839	25.0	21.0	27.0	15.0	28.0
100-109	23.464813160958776	25.0	21.0	27.0	15.4	28.0
110-119	23.452139793447948	25.0	21.0	27.0	16.0	28.0
120-129	23.448080182172095	25.0	21.0	27.0	16.0	28.0
130-139	23.42958569746689	25.0	21.0	27.0	16.0	28.0
140-149	23.379476984190976	25.0	21.0	27.0	16.0	28.0
150-159	23.321631216013774	24.3	21.0	27.0	15.4	28.0
160-169	23.257157769951185	24.0	21.0	27.0	15.0	28.0
170-179	23.17041064436372	24.0	21.0	27.0	15.0	28.0
180-189	23.050395609337546	24.0	21.0	27.0	15.0	28.0
190-199	22.88358513123247	24.0	20.0	27.0	14.2	28.0
200-209	22.689981171333148	24.0	20.0	27.0	14.0	28.0
210-219	22.48309005045893	23.5	20.0	27.0	14.0	28.0
220-229	22.27212353134699	23.0	20.0	26.5	13.7	28.0
230-239	22.03401932862998	23.0	19.4	26.0	13.0	28.0
240-249	21.802090771714894	23.0	19.0	26.0	13.0	28.0
250-259	21.542570680793098	22.9	19.0	26.0	13.0	27.2
260-269	21.214191261304883	22.0	18.1	25.3	12.4	27.0
270-279	20.84864346482195	22.0	18.0	25.0	12.0	27.0
280-289	20.522214786897173	22.0	17.1	25.0	12.0	27.0
290-299	20.12238346676954	21.1	16.1	25.0	12.0	27.0
300-309	19.80675782755271	21.0	15.7	24.0	11.6	27.0
310-319	19.660641707265135	21.0	15.4	24.0	11.2	27.0
320-329	19.49753889491999	20.2	15.0	24.0	11.2	26.7
330-339	19.699540381649122	20.3	15.0	24.4	11.3	27.0
340-349	20.072091314427187	20.333333333333332	13.666666666666666	25.166666666666668	10.166666666666666	27.166666666666668
350-359	21.676735593410164	NaN	NaN	NaN	NaN	NaN
360-369	23.948160173160172	NaN	NaN	NaN	NaN	NaN
370-379	14.539381598793364	NaN	NaN	NaN	NaN	NaN
380-389	5.0	NaN	NaN	NaN	NaN	NaN
390-391	5.0	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
12	3.0
13	14.0
14	561.0
15	8233.0
16	33595.0
17	90020.0
18	182607.0
19	300175.0
20	447453.0
21	629283.0
22	864268.0
23	1163848.0
24	1490563.0
25	1650025.0
26	1146247.0
27	153475.0
28	360.0
29	2.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	22.54961932336462	25.899845749131327	21.43376353983932	30.11677138766473
2	18.37293517297223	26.17064253549804	34.50759074063454	20.94883155089519
3	16.064612831299936	30.364874131389197	31.724837918951387	21.84567511835948
4	17.893272319198815	31.617984759210326	30.98699969561554	19.501743225975318
5	18.941977263804276	32.825106865413545	29.067502768134034	19.165413102648145
6	18.730182537546877	30.92777706705722	31.0228175609737	19.319222834422206
7	19.805269919414094	30.907546038762206	30.16541898447345	19.12176505735025
8	18.456604137962135	29.56168637813373	32.01472612995991	19.96698335394423
9	19.186710211780024	28.790909933079533	31.86965335952706	20.152726495613386
10-19	19.87948875174433	30.354894634451913	29.502610795208078	20.263005818595683
20-29	19.863962628739394	30.403078661633476	29.391999106821004	20.340959602806127
30-39	19.938932913812057	30.491498965291413	29.285438715119767	20.284129405776767
40-49	20.008353818899607	30.47034157711047	29.21567198597881	20.305632618011114
50-59	20.06312402655432	30.43347778433577	29.18264310884876	20.320755080261147
60-69	20.112272756722827	30.424489608305098	29.141497045115738	20.321740589856336
70-79	20.14707166400869	30.422137463693062	29.132175037998074	20.298615834300175
80-89	20.1593925440441	30.42228847230575	29.115629168748065	20.302689814902084
90-99	20.176917662357194	30.432805159561727	29.109269352825617	20.281007825255465
100-109	20.183057274562703	30.46578708249632	29.079407260172026	20.27174838276895
110-119	20.19102601791327	30.492297268297534	29.074375964871823	20.242300748917366
120-129	20.186257351213012	30.513374898435192	29.062238232816362	20.23812951753543
130-139	20.203323159255987	30.566923610398227	29.032738049863482	20.197015180482307
140-149	20.204859418045785	30.58325346735532	29.045111157646524	20.166775956952364
150-159	20.22442470025608	30.604369438308414	29.01556331250016	20.155642548935347
160-169	20.20248865507484	30.641449419624838	29.012366066320126	20.143695858980195
170-179	20.172472435187352	30.678232098897247	29.030733305104246	20.118562160811155
180-189	20.15491762850001	30.691150941452083	29.04904944695999	20.104881983087914
190-199	20.1717128646391	30.70303279762912	29.02678287361942	20.098471464112354
200-209	20.14790652577228	30.753669272221888	29.031098126945654	20.06732607506017
210-219	20.175426384624583	30.72818670584871	29.02475156882001	20.0716353407067
220-229	20.16533886784876	30.806175519398632	29.00566188303965	20.02282372971295
230-239	20.20641617446973	30.781849280589096	28.97312748149094	20.038607063450236
240-249	20.158868835046952	30.834139915303986	28.96615496975563	20.040836279893433
250-259	20.21754318165699	30.882933577120493	28.90603490497494	19.993488336247573
260-269	20.295533816923257	30.85783548541446	28.835006932687314	20.01162376497497
270-279	20.296035316668156	30.96030847460994	28.746064614804002	19.997591593917903
280-289	20.27497210338424	31.1775048278645	28.454548074824693	20.092974993926568
290-299	20.53611329723915	31.203802940001662	28.198942485278238	20.06114127748095
300-309	20.645996235157835	31.61924413553432	27.564798725745725	20.16996090356212
310-319	21.132132663113488	31.736088307358106	27.28788299780242	19.843896031725986
320-329	20.100166944908178	31.903171953255427	27.829716193656097	20.1669449081803
330-339	19.25133689839572	33.532557407989934	27.27272727272727	19.943378420887072
340-349	19.209558823529413	35.11029411764706	24.540441176470587	21.139705882352942
350-359	23.513513513513516	27.297297297297295	25.945945945945947	23.243243243243246
360-369	21.75925925925926	26.851851851851855	25.925925925925924	25.462962962962965
370-379	24.545454545454547	26.36363636363636	28.18181818181818	20.909090909090907
380-389	28.57142857142857	22.448979591836736	18.367346938775512	30.612244897959183
390-391	50.0	0.0	0.0	50.0
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	7.0
1	8.0
2	18.0
3	30.5
4	39.5
5	60.0
6	106.5
7	172.0
8	250.5
9	345.5
10	461.5
11	613.0
12	847.5
13	1225.166666666667
14	1708.1666666666665
15	2286.8333333333326
16	3177.333333333335
17	4491.8333333333385
18	6302.499999999998
19	8577.33333333331
20	11701.833333333369
21	15951.250000000207
22	21082.25000000007
23	28310.333333332637
24	38220.49999999934
25	50445.75000000152
26	66142.75000000499
27	86497.41666666542
28	111597.16666665189
29	141357.99999998213
30	175011.2500000153
31	210451.75000005632
32	246722.08333345418
33	281387.91666676797
34	313700.83333334216
35	343019.41666658135
36	367113.6666664937
37	387299.58333307825
38	404640.0833330145
39	415800.8333329716
40	421618.9166662774
41	421893.49999960634
42	415253.3333329568
43	401323.3333330137
44	379072.2499997612
45	350817.1666665275
46	317643.9166666448
47	282553.5833334392
48	247378.00000013426
49	213198.91666674582
50	182095.25000003274
51	154098.91666666395
52	129012.99999996989
53	107864.49999998277
54	90529.08333332662
55	76017.166666668
56	63972.83333334004
57	53648.0000000037
58	44588.000000001244
59	36834.74999999928
60	30362.49999999886
61	24721.249999999534
62	19981.333333333252
63	15971.750000000244
64	12490.083333333421
65	9613.333333333327
66	7397.499999999962
67	5715.583333333323
68	4317.416666666673
69	3247.6666666666706
70	2493.3333333333335
71	1905.833333333332
72	1432.9999999999995
73	1049.5
74	752.1666666666667
75	540.8333333333334
76	419.1666666666667
77	304.5
78	192.0
79	131.83333333333331
80	109.16666666666666
81	90.0
82	65.5
83	50.0
84	41.5
85	31.5
86	23.5
87	16.0
88	10.5
89	7.5
90	6.5
91	6.5
92	5.5
93	5.0
94	5.5
95	5.0
96	4.0
97	4.0
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-389	0.0
390-391	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-29	33161.0
30-39	77063.0
40-49	92800.0
50-59	109025.0
60-69	127830.0
70-79	156245.0
80-89	214631.0
90-99	323213.0
100-109	490011.0
110-119	668028.0
120-129	779659.0
130-139	809826.0
140-149	773494.0
150-159	698671.0
160-169	598434.0
170-179	492337.0
180-189	397740.0
190-199	316901.0
200-209	249763.0
210-219	193769.0
220-229	148929.0
230-239	114175.0
240-249	85932.0
250-259	64109.0
260-269	47544.0
270-279	35991.0
280-289	27340.0
290-299	18031.0
300-309	9884.0
310-319	4200.0
320-329	1461.0
330-339	382.0
340-349	94.0
350-359	33.0
360-369	9.0
370-379	10.0
380-389	6.0
390-392	1.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	95.23998402038804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	95.71632866993131	91.16021613014468
2	3.779799694503468	7.199761250095557
3	0.3806955743344945	1.0877232124874914
4	0.0835044830081008	0.3181186250928914
5	0.01875332046404243	0.08930329706623084
6	0.010126813044395213	0.05786865075153943
7	0.006397676455020736	0.04265202223406514
8	0.0015119343973964007	0.011519728627832657
9	0.0013529058405113814	0.011596565756825423
>10	0.0015135116514361825	0.01855361143173331
>50	0.0	0.0
>100	1.541636981504125E-5	0.0026869063111515656
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.450760544519781E-5	0.0
2	0.0	0.0	0.0	2.450760544519781E-5	0.0
3	0.0	0.0	0.0	2.450760544519781E-5	0.0
4	0.0	0.0	0.0	2.450760544519781E-5	0.0
5	0.0	2.450760544519781E-5	0.0	2.450760544519781E-5	0.0
6	0.0	4.901521089039562E-5	0.0	2.450760544519781E-5	0.0
7	0.0	4.901521089039562E-5	1.2253802722598905E-5	2.450760544519781E-5	0.0
8	0.0	4.901521089039562E-5	1.2253802722598905E-5	2.450760544519781E-5	1.2253802722598905E-5
9	0.0	4.901521089039562E-5	1.2253802722598905E-5	3.676140816779671E-5	1.2253802722598905E-5
10-19	2.450760544519781E-6	5.391673197943517E-5	1.7155323811638464E-5	3.676140816779671E-5	1.2253802722598905E-5
20-29	3.79867884400566E-5	6.617053470203409E-5	2.450760544519781E-5	4.656445034587583E-5	2.6958365989717592E-5
30-39	9.190352041949177E-5	9.557966123627144E-5	3.4310647623276934E-5	4.901521089039562E-5	4.043754898457638E-5
40-49	9.92558020530511E-5	1.3111568913180827E-4	4.901521089039562E-5	5.391673197943517E-5	7.229743606333354E-5
50-59	1.5194715376022643E-4	1.7155323811638467E-4	6.12690136129945E-5	9.312890069175166E-5	8.577661905819234E-5
60-69	1.67877097299605E-4	2.0954002655644124E-4	8.087509796915278E-5	1.2376340749824892E-4	8.577661905819234E-5
70-79	1.7155323811638467E-4	2.7693594153073523E-4	8.577661905819234E-5	1.421441115821473E-4	1.0293194286983079E-4
80-89	1.9238470274480279E-4	3.2227501160435123E-4	8.577661905819234E-5	1.923847027448028E-4	1.22538027225989E-4
90-99	2.0831464628418136E-4	3.737409830392666E-4	9.680504150853133E-5	2.4017453336293849E-4	1.22538027225989E-4
100-109	2.1811768846226052E-4	4.0805163066254355E-4	9.803042178079124E-5	2.5120295581327755E-4	1.2866492858728848E-4
110-119	2.867389837088143E-4	4.166292925683627E-4	1.2008726668146924E-4	2.5855523744683687E-4	1.5929943539378578E-4
120-129	3.198242510598314E-4	4.166292925683627E-4	1.3479182994858795E-4	2.9654202588689344E-4	1.5929943539378578E-4
130-139	3.455572367772891E-4	4.252069544741819E-4	1.4336949185440718E-4	3.0757044833723253E-4	1.5929943539378578E-4
140-149	3.6638870140570716E-4	4.3991151774130065E-4	1.4704563267118684E-4	3.1859887078757157E-4	1.5929943539378578E-4
150-159	3.725156027670067E-4	4.411368980135605E-4	1.4704563267118684E-4	3.1859887078757157E-4	1.5929943539378578E-4
160-169	3.7986788440056606E-4	4.4236227828582046E-4	1.6297557621056542E-4	3.1859887078757157E-4	1.5929943539378578E-4
170-179	3.7986788440056606E-4	4.5339070073615944E-4	1.7277861838864455E-4	3.345288143269501E-4	1.5929943539378578E-4
180-189	3.8354402521734567E-4	4.5339070073615944E-4	1.9606084356158247E-4	3.4310647623276934E-4	1.5929943539378578E-4
190-199	3.9212168712316495E-4	4.5339070073615944E-4	1.9606084356158247E-4	3.4310647623276934E-4	1.5929943539378578E-4
200-209	3.9212168712316495E-4	4.5339070073615944E-4	1.9606084356158247E-4	3.4310647623276934E-4	1.5929943539378578E-4
210-219	3.9212168712316495E-4	4.5339070073615944E-4	1.9606084356158247E-4	3.4310647623276934E-4	1.5929943539378578E-4
220-229	3.9212168712316495E-4	4.5339070073615944E-4	1.985116041061022E-4	3.4310647623276934E-4	1.5929943539378578E-4
230-239	3.9212168712316495E-4	4.5339070073615944E-4	2.0831464628418136E-4	3.4310647623276934E-4	1.5929943539378578E-4
240-249	3.9212168712316495E-4	4.5339070073615944E-4	2.0831464628418136E-4	3.4310647623276934E-4	1.5929943539378578E-4
250-259	3.9212168712316495E-4	4.5339070073615944E-4	2.0831464628418136E-4	3.4310647623276934E-4	1.5929943539378578E-4
260-269	3.9212168712316495E-4	4.5339070073615944E-4	2.0831464628418136E-4	3.4310647623276934E-4	1.5929943539378578E-4
270-279	3.9212168712316495E-4	4.5339070073615944E-4	2.0831464628418136E-4	3.4310647623276934E-4	1.5929943539378578E-4
280-289	3.9212168712316495E-4	4.5339070073615944E-4	2.0831464628418136E-4	3.4310647623276934E-4	1.5929943539378578E-4
290-299	3.9212168712316495E-4	4.5339070073615944E-4	2.0831464628418136E-4	3.4310647623276934E-4	1.5929943539378578E-4
300-309	3.9212168712316495E-4	4.5339070073615944E-4	2.0831464628418136E-4	3.4310647623276934E-4	1.5929943539378578E-4
310-319	3.9212168712316495E-4	4.5339070073615944E-4	2.0831464628418136E-4	3.4310647623276934E-4	1.5929943539378578E-4
320-329	3.9212168712316495E-4	4.5339070073615944E-4	2.0831464628418136E-4	3.4310647623276934E-4	1.5929943539378578E-4
330-339	3.9212168712316495E-4	4.5339070073615944E-4	2.0831464628418136E-4	3.4310647623276934E-4	1.5929943539378578E-4
340-349	4.056008701180238E-4	4.668698837310183E-4	2.0954002655644124E-4	3.443318565050292E-4	1.5929943539378578E-4
350-359	4.166292925683627E-4	4.778983061813573E-4	2.2056844900678025E-4	3.5536027895536823E-4	1.5929943539378578E-4
360-369	4.166292925683627E-4	4.778983061813573E-4	2.2056844900678025E-4	3.5536027895536823E-4	1.5929943539378578E-4
370-379	4.166292925683627E-4	4.864759680871764E-4	2.2056844900678025E-4	3.5536027895536823E-4	1.5929943539378578E-4
380	4.2888309529096167E-4	5.024059116265551E-4	2.328222517293792E-4	3.676140816779671E-4	1.7155323811638464E-4
>>END_MODULE
