##FastQC	0.11.8
>>Basic Statistics	pass
#Measure	Value
Filename	SPD2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1172322
Sequences flagged as poor quality	0
Sequence length	25-384
%GC	49
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.29938105742279	27.0	24.0	28.0	20.0	29.0
2	20.19518186982757	21.0	19.0	23.0	14.0	24.0
3	24.88739271292358	26.0	23.0	27.0	20.0	28.0
4	25.13861805886096	26.0	24.0	27.0	20.0	28.0
5	24.846549838696195	26.0	23.0	27.0	20.0	28.0
6	24.809004693249808	26.0	23.0	27.0	20.0	28.0
7	23.988793181395554	25.0	22.0	27.0	20.0	28.0
8	25.075854586026708	26.0	23.0	28.0	20.0	29.0
9	19.804175815177057	21.0	17.0	23.0	14.0	24.0
10-19	25.04536373112507	25.9	24.0	27.1	20.5	28.4
20-29	24.723996096581796	25.5	23.6	27.1	20.1	28.1
30-39	24.012884327810546	25.1	22.6	26.3	19.5	27.0
40-49	23.82577527887758	24.8	22.2	26.7	18.4	27.7
50-59	22.990388290890117	23.9	20.7	26.2	16.9	27.7
60-69	23.801026865300173	24.7	21.6	27.0	17.9	28.0
70-79	22.236953067786974	23.3	19.6	26.1	14.7	27.3
80-89	21.945488066028773	22.9	19.1	25.8	13.6	27.5
90-99	22.767380803632935	23.7	20.1	26.3	16.1	27.5
100-109	22.872185087947837	23.8	20.3	26.5	15.8	27.8
110-119	22.547570537173904	23.7	19.8	26.5	14.4	27.8
120-129	23.48172623075745	24.5	21.2	26.7	16.9	27.9
130-139	22.47158754214354	23.7	19.6	26.6	14.1	28.1
140-149	23.97924659302414	25.6	22.0	27.0	16.9	28.1
150-159	23.549949510144835	24.7	21.5	27.0	17.1	28.0
160-169	23.18253116647469	24.0	21.1	26.6	16.8	27.9
170-179	21.95499568501404	23.0	19.0	26.1	13.3	27.8
180-189	23.209765367006035	24.3	21.0	27.0	15.1	28.0
190-199	21.897037974831203	23.3	19.1	26.1	12.9	27.5
200-209	20.51959245280925	21.7	16.7	25.0	11.5	27.2
210-219	22.059928172621404	23.0	19.6	26.3	13.7	28.0
220-229	22.11429715740374	23.1	19.7	26.0	13.7	28.0
230-239	22.68555108725981	23.8	20.1	26.9	13.4	28.0
240-249	19.766784716740847	20.9	15.6	24.4	11.7	26.4
250-259	17.73735703343291	18.5	13.4	21.8	10.1	24.9
260-269	18.025342929176297	18.5	13.9	21.8	10.3	25.3
270-279	18.404686109605578	18.333333333333332	13.0	21.0	9.0	25.333333333333332
280-289	20.125133769640826	NaN	NaN	NaN	NaN	NaN
290-299	20.95064734380524	NaN	NaN	NaN	NaN	NaN
300-309	23.06274778327273	NaN	NaN	NaN	NaN	NaN
310-319	26.03571428571429	NaN	NaN	NaN	NaN	NaN
320-329	26.33962703962704	NaN	NaN	NaN	NaN	NaN
330-339	25.55454545454546	NaN	NaN	NaN	NaN	NaN
340-349	24.398888888888887	NaN	NaN	NaN	NaN	NaN
350-359	24.973015873015875	NaN	NaN	NaN	NaN	NaN
360-369	24.773809523809526	NaN	NaN	NaN	NaN	NaN
370-379	12.813333333333333	NaN	NaN	NaN	NaN	NaN
380-384	5.0	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
13	1.0
14	17.0
15	532.0
16	2317.0
17	6911.0
18	17915.0
19	43261.0
20	93308.0
21	166989.0
22	247357.0
23	280904.0
24	209313.0
25	88662.0
26	14687.0
27	148.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	99.78563909915536	0.001791316720150266	0.2117165761625219	8.530079619763171E-4
2	99.35248165606377	0.004094438217486322	0.005971055733834219	0.6374528499849017
3	0.4695808830679626	0.713114656212201	0.044015210837977964	98.77328924988186
4	1.4559992902973755	97.94390960845229	0.00801827484257738	0.5920728264077617
5	97.88027521448885	0.6017971171742917	0.004862145383265007	1.5130655229535912
6	0.7602006957132937	1.5552894170714189	0.04290630048740875	97.64160358672788
7	0.09613399731473093	97.52354728479035	1.6225917452713505	0.7577269726235625
8	0.06909364492008169	0.8498518325170047	98.861831476335	0.2192230462279135
9	0.034973326441029004	0.05177758329196245	96.90656662589289	3.006682464374123
10-19	20.032192520484987	30.517144607027763	19.30667512850565	30.143987743981604
20-29	14.362421503558773	19.97374183988241	11.503598888936024	54.160237767622796
30-39	23.70238086643282	12.192181057650227	24.5288839016052	39.57655417431175
40-49	34.585269945876234	45.43730599344503	7.284888378137959	12.692535682540779
50-59	66.56196718503841	2.2802425255674255	6.262969660741853	24.894820628652308
60-69	31.11066650656131	19.783644808049843	25.88478641772073	23.220902267668126
70-79	2.571663921072205	19.333765616486247	46.44265146948266	31.651918992958883
80-89	39.7511359130923	28.03986000623479	19.22446918469504	12.984534895977868
90-99	32.32983138566899	20.106395056055838	29.360728351930398	18.203045206344775
100-109	39.976370751019864	24.451406752612673	11.543858372054034	24.028364124313434
110-119	12.049602619026677	20.34650902691087	27.103670074941157	40.5002182791213
120-129	19.888421225248372	20.180469438774644	44.630692041422186	15.300417294554796
130-139	32.65334545365331	43.753569050714994	5.626949494812768	17.966136000818924
140-149	12.563424461045772	29.627817750063723	21.095664436598398	36.713093352292105
150-159	32.721033615235754	11.214921804366856	20.799022816764897	35.26502176363249
160-169	41.73286502859177	30.313175696881657	17.007071113105017	10.946888161421565
170-179	7.0269129641412285	58.30518060036559	22.565717582988086	12.10218885250509
180-189	12.253682126037118	25.358806726418948	57.43937625462879	4.94813489291514
190-199	18.067248521541174	20.181428669086245	38.64853111403656	23.102791695336027
200-209	9.952067331854371	21.988539258830592	32.87797593679393	35.18141747252111
210-219	25.171925535685265	29.782720813122083	39.28047563571623	5.7648780154764285
220-229	27.48470107503982	13.165861060793201	46.54865654907517	12.800781315091811
230-239	9.330750554008407	34.90937727478467	38.21021466888387	17.549657502323058
240-249	3.86112467040699	26.576705560854457	41.55917151907912	28.002998249659427
250-259	6.229323004922134	26.57145162591786	26.716694908416038	40.48253046074397
260-269	16.878612716763005	37.16763005780347	23.121387283236995	22.832369942196532
270-279	27.34463276836158	31.751412429378533	20.903954802259886	20.0
280-289	22.39089184060721	29.98102466793169	13.472485768500949	34.15559772296015
290-299	35.62945368171022	30.878859857482183	13.064133016627078	20.42755344418052
300-309	36.5296803652968	27.397260273972602	20.091324200913242	15.981735159817351
310-319	25.563909774436087	24.81203007518797	24.06015037593985	25.563909774436087
320-329	25.203252032520325	22.76422764227642	26.01626016260163	26.01626016260163
330-339	24.545454545454547	25.454545454545453	24.545454545454547	25.454545454545453
340-349	23.91304347826087	26.08695652173913	27.173913043478258	22.82608695652174
350-359	25.0	26.190476190476193	22.61904761904762	26.190476190476193
360-369	24.59016393442623	24.59016393442623	26.229508196721312	24.59016393442623
370-379	23.809523809523807	23.809523809523807	28.57142857142857	23.809523809523807
380-384	50.0	16.666666666666664	0.0	33.33333333333333
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.5
14	1.0
15	1.0
16	1.0
17	1.0
18	1.0
19	1.0
20	0.5
21	0.5
22	1.0
23	1.5
24	3.0
25	4.0
26	2.5
27	1.5
28	1.5
29	1.0
30	1.0
31	1.0
32	1.5
33	1.5
34	2.0
35	3.0
36	2.0
37	2.0
38	3.5
39	4.666666666666666
40	3.0
41	2.5
42	3.416666666666667
43	12.583333333333336
44	49.666666666666735
45	49.16666666666673
46	190.66666666666748
47	466.3333333333259
48	5395.416666666238
49	54382.08333344227
50	102519.74999965527
51	108061.49999957916
52	60090.00000014255
53	73226.58333340693
54	28359.749999981534
55	34121.41666666551
56	96308.99999999996
57	104756.33333333331
58	52188.5
59	31876.5
60	29211.0
61	27357.5
62	21108.0
63	13371.5
64	7681.0
65	6268.0
66	3133.5
67	1064.5
68	418.5
69	97.5
70	24.0
71	7.5
72	2.0
73	3.5
74	4.0
75	2.5
76	2.0
77	2.5
78	3.5
79	2.5
80	1.0
81	1.0
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
380-384	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-29	4114.0
30-39	10428.0
40-49	15244.0
50-59	13038.0
60-69	19396.0
70-79	23700.0
80-89	34584.0
90-99	19465.0
100-109	28970.0
110-119	25835.0
120-129	24770.0
130-139	19725.0
140-149	19736.0
150-159	17186.0
160-169	19531.0
170-179	13019.0
180-189	20839.0
190-199	32289.0
200-209	40370.0
210-219	22649.0
220-229	22861.0
230-239	53301.0
240-249	666959.0
250-259	4080.0
260-269	116.0
270-279	55.0
280-289	17.0
290-299	13.0
300-309	18.0
310-319	1.0
320-329	2.0
330-339	1.0
340-349	1.0
350-359	2.0
360-369	1.0
370-379	4.0
380-385	2.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	4.594812687981629
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.18909887498607	3.638590762606178
2	8.573125905023577	0.7878381536813264
3	3.274793004863922	0.451411813477867
4	1.8731667471132067	0.34427401345364156
5	1.0284780752237033	0.23628320546743983
6	0.7370140719563361	0.20318649654275872
7	0.5569375858612111	0.17913167201502658
8	0.4344113169717447	0.15968309048196655
9	0.36200943080978726	0.14970289732684364
>10	2.819960643077266	2.749927067819251
>50	0.49381799279694055	1.6167059903337138
>100	0.4956744514164779	4.946849073889256
>500	0.05569375858612112	1.7783509991282258
>1k	0.08539709649871904	7.998485057859531
>5k	0.0037129172390747414	0.9204808917686438
>10k+	0.016708127575836337	73.83909881414833
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAG	314382	26.81703491020385	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAG	175941	15.00790738380752	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAG	118791	10.13296688111287	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAG	55917	4.769764620982972	No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAG	55611	4.743662577346496	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAG	55221	4.710395266829421	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAAGCAG	42237	3.60284972899937	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCTGAAGAAGCAG	37355	3.186411241962533	No Hit
GGCAGCATTCCGAGTAACTCCTCAGCCCGGAGTTCCACCCGAGGAAGCGG	10177	0.8681062029032979	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCAGGAGTTCCGCCCGAAGAAGCAG	5786	0.49355040679949713	No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAGAAGCAGG	5005	0.4269304849691467	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCCGAGGAAGCAG	4806	0.40995562652581796	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAAGAAGCAG	4397	0.3750676008809866	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCCGAGGAAGCAG	4243	0.36193127826655136	No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAGCAGG	3746	0.3195367825563284	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCGG	3676	0.3135657268224941	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAGCAGG	3385	0.28874319512898333	No Hit
GGCAGCATTCCGAGTACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGG	3088	0.2634088586582867	No Hit
GGCAGCATTCCGAGTAACTCCTCACCTGGAGTTCCGCCCGAGGAAGCAGG	3017	0.2573525021282549	No Hit
GGCAGCATCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGG	2870	0.244813285087203	No Hit
GGCAGCATTCCGAGTAACTCCGCACCCGGAGTTCCTCCTGAAGAAGCAGG	2674	0.2280943290324672	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGAAGCAGG	2564	0.21871124145072773	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCGGGAGTTCCACCCGAGGAAGCAG	2510	0.21410499845605557	No Hit
GGCGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGG	2378	0.2028452933579682	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCTGAGGAAGCGG	2265	0.19320630338763584	No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCGG	2262	0.19295040099904293	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAGCAGG	2225	0.18979427153973055	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCCCCTGAAGAAGCAG	2195	0.1872352476538016	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGT	2071	0.17665794892529527	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGGCTGAAGAAGCAG	1907	0.1626686183488837	No Hit
GGCAGCATCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGG	1873	0.1597683912781642	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAGCAGG	1829	0.1560151562454684	No Hit
GGCAGCATTCCGAGTACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGG	1810	0.1543944411177134	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCCA	1789	0.15260312439756313	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCCGCTGAAGAAGCAG	1759	0.15004410051163417	No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGGAGTTCCTCCTGAAGAAGCA	1722	0.1468879710523218	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAG	1693	0.14441424796259048	No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGT	1566	0.13358104684549127	No Hit
GGCAGCATTCCGAGTACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGG	1543	0.13161912853294572	No Hit
GGCAGCATCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGG	1384	0.1180563019375223	No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAGAGCAGGG	1364	0.11635028601356964	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCTCCTGAAGAAGCAG	1360	0.11600908282877913	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAGCAGG	1353	0.11541197725539572	No Hit
GGCAGCATTCCGAGTAACTCCTCACCTGGAGTTCCACCTGAAGAAGCAGG	1284	0.1095262223177591	No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCCA	1273	0.10858791355958516	No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGAAGCAGG	1267	0.10807610878239937	No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAAGCA	1253	0.10688189763563252	No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCCGGAGTTCCTCCTGAAGAAGCA	1232	0.10509058091548226	No Hit
GGCAGCATCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAGG	1188	0.10133734588278648	No Hit
GGCAGCATTCCGAGTACTCCTCAACCCGGAGTTCCGCCTGAAGAAGCAGG	1181	0.10074024030940305	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0
320-329	0.0	0.0	0.0	0.0	0.0
330-339	0.0	0.0	0.0	0.0	0.0
340-349	0.0	0.0	0.0	0.0	0.0
350-359	0.0	0.0	0.0	0.0	0.0
360-369	0.0	0.0	0.0	0.0	0.0
370-372	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
