FastQCFastQC Report
Mon 14 Oct 2019
SPD2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSPD2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1172322
Sequences flagged as poor quality0
Sequence length25-384
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAG31438226.81703491020385No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAG17594115.00790738380752No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAG11879110.13296688111287No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAAGAAGCAG559174.769764620982972No Hit
GGCAGCATTCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAG556114.743662577346496No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAG552214.710395266829421No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAAGCAG422373.60284972899937No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCTGAAGAAGCAG373553.186411241962533No Hit
GGCAGCATTCCGAGTAACTCCTCAGCCCGGAGTTCCACCCGAGGAAGCGG101770.8681062029032979No Hit
GGCAGCATTCCGAGTAACTCCTCAACCAGGAGTTCCGCCCGAAGAAGCAG57860.49355040679949713No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAGAAGCAGG50050.4269304849691467No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCCGAGGAAGCAG48060.40995562652581796No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAAGAAGCAG43970.3750676008809866No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCCGAGGAAGCAG42430.36193127826655136No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAGCAGG37460.3195367825563284No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCGG36760.3135657268224941No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAGCAGG33850.28874319512898333No Hit
GGCAGCATTCCGAGTACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGG30880.2634088586582867No Hit
GGCAGCATTCCGAGTAACTCCTCACCTGGAGTTCCGCCCGAGGAAGCAGG30170.2573525021282549No Hit
GGCAGCATCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGG28700.244813285087203No Hit
GGCAGCATTCCGAGTAACTCCGCACCCGGAGTTCCTCCTGAAGAAGCAGG26740.2280943290324672No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGAAGCAGG25640.21871124145072773No Hit
GGCAGCATTCCGAGTAACTCCTCAACCGGGAGTTCCACCCGAGGAAGCAG25100.21410499845605557No Hit
GGCGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGG23780.2028452933579682No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCGCCTGAGGAAGCGG22650.19320630338763584No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCGG22620.19295040099904293No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAGCAGG22250.18979427153973055No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCCCCTGAAGAAGCAG21950.1872352476538016No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGT20710.17665794892529527No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGGCTGAAGAAGCAG19070.1626686183488837No Hit
GGCAGCATCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGG18730.1597683912781642No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAGCAGG18290.1560151562454684No Hit
GGCAGCATTCCGAGTACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGG18100.1543944411177134No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCCA17890.15260312439756313No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCCGCTGAAGAAGCAG17590.15004410051163417No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGGAGTTCCTCCTGAAGAAGCA17220.1468879710523218No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCCGAGGAAGCAGGGGCCGCGGTAG16930.14441424796259048No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCAGGGGCTGCGGT15660.13358104684549127No Hit
GGCAGCATTCCGAGTACTCCTCAACCTGGAGTTCCACCTGAAGAAGCAGG15430.13161912853294572No Hit
GGCAGCATCCGAGTAACACCGCAACCCGGAGTTCCTCCTGAAGAAGCAGG13840.1180563019375223No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAGAGCAGGG13640.11635028601356964No Hit
GGCAGCATTCCGAGTAACTCCTCAACCCGGAGTTCCTCCTGAAGAAGCAG13600.11600908282877913No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGGAGCAGG13530.11541197725539572No Hit
GGCAGCATTCCGAGTAACTCCTCACCTGGAGTTCCACCTGAAGAAGCAGG12840.1095262223177591No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAGCCA12730.10858791355958516No Hit
GGCAGCATTCCGAGTAACTCCTCAACCTGGAGTTCCGCCTGAGAAGCAGG12670.10807610878239937No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCGGAGTTCCTCCTGAAGAAAGCA12530.10688189763563252No Hit
GGCAGCATTCCGAGTAACTCCGCAACCCCGGAGTTCCTCCTGAAGAAGCA12320.10509058091548226No Hit
GGCAGCATCCGAGTAACTCCTCAACCTGGAGTTCCACCTGAGGAAGCAGG11880.10133734588278648No Hit
GGCAGCATTCCGAGTACTCCTCAACCCGGAGTTCCGCCTGAAGAAGCAGG11810.10074024030940305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)