FastQCFastQC Report
Mon 14 Oct 2019
SPD1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSPD1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1953579
Sequences flagged as poor quality0
Sequence length25-379
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGT123729763.33488433280661No Hit
ATGTCACCACAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTG311051.5922058949241364No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCGGGTGT306261.5676867943400292No Hit
ATGTCACCACAAACAGAGACTAAAGCGAGTGTTGGATTCAAGGCCGGTGT237761.2170482995568646No Hit
ATGTCACCACAAACAGAGACTAAAGCAGGTGTTGGATTCAAAGCTGGTGT196791.0073306480055324No Hit
ATGTCACCACAAACAGAGACTAAAAGCAAGTGTTGGATTCAAAGCTGGTG193520.9905921388385112No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAGCTGGTGTG154700.7918799290942419No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGTTCAAAGCTGGTGTG120200.6152809791669547No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCCGGTGT118660.6073980115470119No Hit
ATGTCACCACAAACAGAGACTAAAGCAGTGTTGGATTCAAAGCTGGTGTG103860.531639621433277No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGGTGGATTCAAAGCTGGTGT94060.48147528203364187No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTAG93020.4761517194851091No Hit
ATGTCACCACAAACAGAGACTAAAGCAAAGTGTTGGATTCAAAGCTGGTG92760.47482082884797594No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGCTGGATTCAAAGCTGGTGT84940.4347917335311241No Hit
ATGTCACCACAAACAGAGACTAAGCAAGTGTTGGATTCAAAGCTGGTGTG83090.42532193476690733No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAAGCTGGTG78160.4000862007628051No Hit
ATGTCACCACAAACAGAGAACTAAAGCAAGTGTTGGATTCAAAGCTGGTG72890.3731100713101441No Hit
ATGTCACCACAAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTG67630.3461851299589113No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGTTCAAGCTGGTGTGA53200.2723206995980198No Hit
ATGTCACCACAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTT50360.25778327879241125No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTTGGATTCAAAGCTGGTG47140.2413007101325311No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTATTAT45950.2352093260625754No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAGTGTTAAAG45210.23142140655688864No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGGGT42900.21959695512697464No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGCTGGATTCAAAGCGGGCGT41430.21207230421702936No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTTGTTGGATTCAAAGCTGGTG37970.19436122112287244No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTACAAAGCTGGTG34100.17455142587015932No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCGGTGTG28370.1452206437518012No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATCAAAGCTGGTGTG27850.14255886247753483No Hit
ATGGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTG27550.14102321943468885No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAGCTGGTGTT26470.13549490448044332No Hit
ATGTCAACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTG26340.13482945916187672No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTT25490.13047847054047979No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTGGATTCAAAGCTGGTGTG24820.12704886774479046No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGT23430.11993372164627078No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGGATTCAAAGCTGGTG22870.11706718796629162No Hit
ATGTCACACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTG22750.11645293074915321No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTTCAAAGCTGGTG22290.11409827808345607No Hit
ATGTCACCAACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTG22060.11292095175060748No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTATT20920.10708550818779278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)