##FastQC	0.11.8
>>Basic Statistics	pass
#Measure	Value
Filename	SPD1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1953579
Sequences flagged as poor quality	0
Sequence length	25-379
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.736424275649973	27.0	25.0	28.0	22.0	29.0
2	25.679552759320202	27.0	24.0	28.0	22.0	29.0
3	25.805946419366713	27.0	25.0	28.0	22.0	29.0
4	25.52519196817738	27.0	24.0	28.0	22.0	29.0
5	25.74132144131361	27.0	24.0	28.0	21.0	29.0
6	25.486787071318847	27.0	24.0	28.0	22.0	28.0
7	26.130598762578835	27.0	25.0	28.0	22.0	29.0
8	21.290889695272114	22.0	20.0	24.0	15.0	24.0
9	25.723718365113466	27.0	25.0	28.0	21.0	29.0
10-19	25.067821214294376	26.5	23.4	27.4	20.1	28.8
20-29	23.907188584561535	25.3	22.1	26.5	18.2	27.6
30-39	23.79358818172375	25.2	22.3	26.5	18.3	27.1
40-49	23.726421554832747	25.0	21.9	26.6	18.3	27.6
50-59	22.771472635082098	23.9	20.6	26.5	15.1	27.7
60-69	20.833739412496918	21.4	18.0	24.6	12.2	26.7
70-79	21.33152881560028	22.7	18.3	25.4	13.0	27.2
80-89	23.444204968282637	24.8	21.3	26.6	15.4	28.0
90-99	22.584672669530296	23.9	19.9	26.3	14.0	28.2
100-109	24.54320279714974	25.9	22.9	27.0	18.3	28.4
110-119	23.562435266339843	24.7	21.7	26.7	17.3	27.7
120-129	23.425332241713235	24.8	21.7	26.7	15.2	27.8
130-139	22.96682935767696	24.0	20.9	26.7	15.5	28.0
140-149	22.91358335675866	24.0	20.7	26.2	16.3	27.6
150-159	22.114702132941375	23.1	19.7	25.9	13.6	27.6
160-169	22.32576325533775	23.2	19.6	26.5	15.2	27.9
170-179	23.49459276528838	24.4	21.4	26.9	17.8	28.0
180-189	22.925664695973637	23.9	20.5	26.4	16.6	27.8
190-199	23.106674503195954	24.2	21.0	26.8	16.8	28.0
200-209	23.266725206775227	24.1	21.0	26.8	16.5	27.9
210-219	22.69329595804387	23.6	20.4	26.3	15.9	27.9
220-229	22.747295162495178	23.5	20.4	26.6	15.8	27.9
230-239	21.57006098906816	22.5	19.2	25.1	13.5	27.2
240-249	19.376300547785885	20.3	14.6	23.7	11.6	26.6
250-259	18.2253509893753	19.1	12.1	22.8	11.0	25.4
260-269	17.692122739829934	18.2	12.5	22.2	9.6	25.7
270-279	19.114484508423555	19.5	14.8	23.6	10.6	26.9
280-289	20.457520299191124	NaN	NaN	NaN	NaN	NaN
290-299	21.146827302117995	NaN	NaN	NaN	NaN	NaN
300-309	21.332498871034478	NaN	NaN	NaN	NaN	NaN
310-319	21.973342918086704	NaN	NaN	NaN	NaN	NaN
320-329	23.343138747662074	NaN	NaN	NaN	NaN	NaN
330-339	23.79070517887909	NaN	NaN	NaN	NaN	NaN
340-349	22.335959595959597	NaN	NaN	NaN	NaN	NaN
350-359	23.392736928104576	NaN	NaN	NaN	NaN	NaN
360-369	22.059444444444445	NaN	NaN	NaN	NaN	NaN
370-379	11.546666666666667	NaN	NaN	NaN	NaN	NaN
>>END_MODULE
>>Per sequence quality scores	warn
#Quality	Count
13	4.0
14	70.0
15	1276.0
16	7004.0
17	21297.0
18	46240.0
19	90284.0
20	167092.0
21	280268.0
22	389690.0
23	421047.0
24	332316.0
25	168013.0
26	28837.0
27	139.0
28	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	0.2142733925784419	99.34192576803908	0.3780241290472512	0.065776710335236
2	0.35724175986740236	0.666264328189441	98.83342316845133	0.1430707434918168
3	98.35169194591056	0.26935178971518425	1.2838999600220926	0.09505630435216594
4	1.782164939324184	0.04427770773539232	97.90256754397953	0.27098980896088665
5	0.17547281169586693	0.4776361744265269	1.8305888832752606	97.51630213060236
6	0.10703432008636457	97.23195222716869	0.29392207840071993	2.3670913743442163
7	0.07319898504232487	2.621905743253792	0.08287353621225453	97.22202173549162
8	0.02492860539553302	1.0523249891609194	0.04366345051825393	98.8790829549253
9	0.012797025357049805	96.15904962123363	0.08748046534079246	3.7406728880685143
10-19	19.92163613552357	59.45653592713681	0.12656258078122257	20.4952653565584
20-29	19.565116240458146	50.20440135226294	10.70245131990759	19.52803108737133
30-39	31.024081652870848	11.110480223653687	48.856268614235226	9.009169509240238
40-49	39.02817013367238	29.146669012936854	20.446538702656607	11.378622150734156
50-59	27.320797674092713	47.94333605383629	24.024789342459023	0.7110769296119756
60-69	10.062325334500786	42.11706790249392	38.09746247676138	9.723144286243917
70-79	0.5956385867823585	29.934479811922742	50.30858224734883	19.161299353946067
80-89	18.739724854443672	37.1484697912668	29.544223060950607	14.567582293338921
90-99	10.921277997827758	60.27498558629698	8.2659659569645	20.53777045891076
100-109	11.530298488369322	27.290361780745165	42.595163075798666	18.584176655086846
110-119	29.527463417829335	18.7887899648698	27.35913668543384	24.324609931867027
120-129	19.91417953041032	35.26119047271209	15.187733360537836	29.636896636339756
130-139	19.24441924216869	22.92292674070378	3.6581516526280256	54.17450236449951
140-149	21.277227408446162	9.885047224371643	30.800744353454373	38.036981013727825
150-159	35.29979485236336	44.511053948424674	6.923729481167238	13.265421718044724
160-169	61.6297274408494	4.733819826083474	12.320514301619621	21.315938431447506
170-179	32.03153110523003	20.052129446054106	26.79117522282	21.12516422589587
180-189	6.2671330107910626	18.592171508951537	46.15215614603494	28.98853933422246
190-199	33.99412781560027	28.97215924529063	21.97926026337757	15.05445267573153
200-209	32.14154440863361	22.0618256483801	28.361047208070744	17.43558273491555
210-219	38.44486585395233	25.143216670224323	12.700580374175786	23.71133710164756
220-229	12.259155442713853	22.213179880164738	24.699996052650082	40.82766862447133
230-239	19.949623942895016	20.041756668863062	42.67043692805179	17.338182460190136
240-249	32.59383771486883	45.622191375211194	2.3127389619237797	19.471231947996184
250-259	55.8577806122449	14.04532967032967	2.39010989010989	27.706779827315543
260-269	28.231101106734197	24.029262802476083	11.57381354342525	36.16582254736447
270-279	26.412429378531073	26.059322033898308	22.88135593220339	24.646892655367232
280-289	25.62076749435666	24.37923250564334	27.539503386004515	22.460496613995485
290-299	26.811594202898554	28.840579710144926	21.304347826086957	23.043478260869566
300-309	23.025210084033613	25.71428571428571	27.899159663865547	23.361344537815125
310-319	26.096997690531175	22.863741339491916	25.635103926096996	25.40415704387991
320-329	26.013513513513516	26.68918918918919	21.95945945945946	25.33783783783784
330-339	24.380165289256198	30.991735537190085	21.487603305785125	23.140495867768596
340-349	24.137931034482758	22.167487684729064	24.137931034482758	29.55665024630542
350-359	25.146198830409354	25.730994152046783	25.146198830409354	23.976608187134502
360-369	22.22222222222222	24.074074074074073	31.48148148148148	22.22222222222222
370-379	26.47058823529412	29.411764705882355	17.647058823529413	26.47058823529412
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	1.0
8	1.0
9	1.0
10	1.0
11	2.0
12	3.0
13	3.0
14	2.0
15	1.5
16	2.0
17	2.5
18	4.0
19	6.0
20	5.5
21	3.0
22	3.0
23	6.0
24	7.0
25	7.0
26	6.0
27	6.0
28	20.0
29	67.5
30	287.5
31	1387.0
32	4990.5
33	19123.0
34	86331.0
35	126712.0
36	88998.83333333333
37	55958.83333333333
38	41692.0
39	43026.166666666635
40	50016.50000000013
41	56515.16666667085
42	63106.333333364964
43	130405.0833328027
44	411086.6666615089
45	108948.66666629177
46	38142.66666664478
47	14002.58333333886
48	6775.333333332122
49	1016.5000000000138
50	235.3333333333332
51	70.83333333333333
52	27.0
53	9.5
54	6.0
55	5.0
56	6.5
57	6.5
58	4.5
59	1.5
60	0.0
61	0.5
62	1.5
63	1.5
64	1.0
65	1.0
66	1.5
67	2.5
68	2.5
69	2.0
70	2.5
71	2.5
72	2.0
73	2.5
74	2.0
75	1.5
76	2.0
77	2.0
78	1.5
79	1.5
80	2.5
81	3.5
82	4.5
83	5.5
84	6.0
85	5.0
86	2.5
87	1.5
88	1.5
89	1.0
90	1.5
91	1.5
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-19	0.0
20-29	0.0
30-39	0.0
40-49	0.0
50-59	0.0
60-69	0.0
70-79	0.0
80-89	0.0
90-99	0.0
100-109	0.0
110-119	0.0
120-129	0.0
130-139	0.0
140-149	0.0
150-159	0.0
160-169	0.0
170-179	0.0
180-189	0.0
190-199	0.0
200-209	0.0
210-219	0.0
220-229	0.0
230-239	0.0
240-249	0.0
250-259	0.0
260-269	0.0
270-279	0.0
280-289	0.0
290-299	0.0
300-309	0.0
310-319	0.0
320-329	0.0
330-339	0.0
340-349	0.0
350-359	0.0
360-369	0.0
370-379	0.0
>>END_MODULE
>>Sequence Length Distribution	warn
#Length	Count
20-29	10132.0
30-39	25216.0
40-49	27187.0
50-59	45634.0
60-69	48892.0
70-79	51467.0
80-89	26180.0
90-99	32703.0
100-109	25340.0
110-119	25929.0
120-129	37727.0
130-139	25308.0
140-149	26632.0
150-159	20117.0
160-169	16941.0
170-179	15853.0
180-189	16362.0
190-199	17695.0
200-209	19036.0
210-219	25069.0
220-229	30779.0
230-239	40438.0
240-249	1144054.0
250-259	197661.0
260-269	1030.0
270-279	99.0
280-289	24.0
290-299	8.0
300-309	16.0
310-319	16.0
320-329	7.0
330-339	5.0
340-349	4.0
350-359	4.0
360-369	8.0
370-379	6.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	5.192077825347841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.3758110718814	4.173174663606629
2	7.713011687025838	0.8009311389371119
3	3.0488596385189837	0.4748974956525735
4	1.7108682633424035	0.35531844688765857
5	1.1179139207579234	0.2902148039307439
6	0.7989384818296789	0.24888904651949664
7	0.5999538036267926	0.2180504788030634
8	0.49396196498605927	0.20517511719754916
9	0.40396889444204037	0.18876941450665202
>10	2.914775562620167	2.9986613345681286
>50	0.3579724361639862	1.3111063789744197
>100	0.32697482297660196	3.607645171796684
>500	0.06499499539290253	2.3452367330882815
>1k	0.05199599631432202	5.499233075413259
>5k	0.009999230060446543	3.9981631959768373
>10k+	0.009999230060446543	73.28453350414091
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGT	1237297	63.33488433280661	No Hit
ATGTCACCACAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTG	31105	1.5922058949241364	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCGGGTGT	30626	1.5676867943400292	No Hit
ATGTCACCACAAACAGAGACTAAAGCGAGTGTTGGATTCAAGGCCGGTGT	23776	1.2170482995568646	No Hit
ATGTCACCACAAACAGAGACTAAAGCAGGTGTTGGATTCAAAGCTGGTGT	19679	1.0073306480055324	No Hit
ATGTCACCACAAACAGAGACTAAAAGCAAGTGTTGGATTCAAAGCTGGTG	19352	0.9905921388385112	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAGCTGGTGTG	15470	0.7918799290942419	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGTTCAAAGCTGGTGTG	12020	0.6152809791669547	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCCGGTGT	11866	0.6073980115470119	No Hit
ATGTCACCACAAACAGAGACTAAAGCAGTGTTGGATTCAAAGCTGGTGTG	10386	0.531639621433277	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGGTGGATTCAAAGCTGGTGT	9406	0.48147528203364187	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTAG	9302	0.4761517194851091	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAAGTGTTGGATTCAAAGCTGGTG	9276	0.47482082884797594	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGCTGGATTCAAAGCTGGTGT	8494	0.4347917335311241	No Hit
ATGTCACCACAAACAGAGACTAAGCAAGTGTTGGATTCAAAGCTGGTGTG	8309	0.42532193476690733	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAAGCTGGTG	7816	0.4000862007628051	No Hit
ATGTCACCACAAACAGAGAACTAAAGCAAGTGTTGGATTCAAAGCTGGTG	7289	0.3731100713101441	No Hit
ATGTCACCACAAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTG	6763	0.3461851299589113	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGTTCAAGCTGGTGTGA	5320	0.2723206995980198	No Hit
ATGTCACCACAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTT	5036	0.25778327879241125	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTTGGATTCAAAGCTGGTG	4714	0.2413007101325311	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTATTAT	4595	0.2352093260625754	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAGTGTTAAAG	4521	0.23142140655688864	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGGGT	4290	0.21959695512697464	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGCTGGATTCAAAGCGGGCGT	4143	0.21207230421702936	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTTGTTGGATTCAAAGCTGGTG	3797	0.19436122112287244	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTACAAAGCTGGTG	3410	0.17455142587015932	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCGGTGTG	2837	0.1452206437518012	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATCAAAGCTGGTGTG	2785	0.14255886247753483	No Hit
ATGGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTG	2755	0.14102321943468885	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAGCTGGTGTT	2647	0.13549490448044332	No Hit
ATGTCAACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTG	2634	0.13482945916187672	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTT	2549	0.13047847054047979	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTGGATTCAAAGCTGGTGTG	2482	0.12704886774479046	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGT	2343	0.11993372164627078	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGGATTCAAAGCTGGTG	2287	0.11706718796629162	No Hit
ATGTCACACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTG	2275	0.11645293074915321	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTTCAAAGCTGGTG	2229	0.11409827808345607	No Hit
ATGTCACCAACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTG	2206	0.11292095175060748	No Hit
ATGTCACCACAAACAGAGACTAAAGCAAGTGTTGGATTCAAAGCTGGTGTGAAAGAGTATAAATTGACTTATT	2092	0.10708550818779278	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-19	0.0	0.0	0.0	0.0	0.0
20-29	0.0	0.0	0.0	0.0	0.0
30-39	0.0	0.0	0.0	0.0	0.0
40-49	0.0	0.0	0.0	0.0	0.0
50-59	0.0	0.0	0.0	0.0	0.0
60-69	0.0	0.0	0.0	0.0	0.0
70-79	0.0	0.0	0.0	0.0	0.0
80-89	0.0	0.0	0.0	0.0	0.0
90-99	0.0	0.0	0.0	0.0	0.0
100-109	0.0	0.0	0.0	0.0	0.0
110-119	0.0	0.0	0.0	0.0	0.0
120-129	0.0	0.0	0.0	0.0	0.0
130-139	0.0	0.0	0.0	0.0	0.0
140-149	0.0	0.0	0.0	0.0	0.0
150-159	0.0	0.0	0.0	0.0	0.0
160-169	0.0	0.0	0.0	0.0	0.0
170-179	0.0	0.0	0.0	0.0	0.0
180-189	0.0	0.0	0.0	0.0	0.0
190-199	0.0	0.0	0.0	0.0	0.0
200-209	0.0	0.0	0.0	0.0	0.0
210-219	0.0	0.0	0.0	0.0	0.0
220-229	0.0	0.0	0.0	0.0	0.0
230-239	0.0	0.0	0.0	0.0	0.0
240-249	0.0	0.0	0.0	0.0	0.0
250-259	0.0	0.0	0.0	0.0	0.0
260-269	0.0	0.0	0.0	0.0	0.0
270-279	0.0	0.0	0.0	0.0	0.0
280-289	0.0	0.0	0.0	0.0	0.0
290-299	0.0	0.0	0.0	0.0	0.0
300-309	0.0	0.0	0.0	0.0	0.0
310-319	0.0	0.0	0.0	0.0	0.0
320-329	0.0	0.0	0.0	0.0	0.0
330-339	0.0	0.0	0.0	0.0	0.0
340-349	0.0	0.0	0.0	0.0	0.0
350-359	0.0	0.0	0.0	0.0	0.0
360-367	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
