The following samples were sequenced using the Ion Proton: KLPNC1-ecoli-res (1453369 reads, Mean Read Length: 130 bp) KLPNC2-Trs502 (1091825 reads, Mean Read Length: 131 bp) KLPNC3-Trs8 (1434440 reads, Mean Read Length: 133 bp) All reads were aligned to the provided reference assemblies: WG5 - the recipient E.coli (4522677 bp) Trs502 (100886 bp) Trs8 (93387 bp) using bwa (version 0.7.16a) with default parameters. samtools flagstat KLP1-vs-pTrc502-29330-42050.bam 0 + 0 in total (QC-passed reads + QC-failed reads) 0 + 0 duplicates 0 + 0 mapped (-nan%:-nan%) samtools flagstat KLP2-vs-pTrc502-29330-42050.bam 143 + 0 in total (QC-passed reads + QC-failed reads) 0 + 0 duplicates 143 + 0 mapped (100.00%:-nan%) samtools flagstat KLP3-vs-pTrc502-29330-42050.bam 0 + 0 in total (QC-passed reads + QC-failed reads) 0 + 0 duplicates 0 + 0 mapped (-nan%:-nan%) samtools flagstat KLP1-vs-pTrc8-23000-26950.bam 0 + 0 in total (QC-passed reads + QC-failed reads) 0 + 0 duplicates 0 + 0 mapped (-nan%:-nan%) samtools flagstat KLP2-vs-pTrc8-23000-26950.bam 0 + 0 in total (QC-passed reads + QC-failed reads) 0 + 0 duplicates 0 + 0 mapped (-nan%:-nan%) samtools flagstat KLP3-vs-pTrc8-23000-26950.bam 23 + 0 in total (QC-passed reads + QC-failed reads) 0 + 0 duplicates 23 + 0 mapped (100.00%:-nan%) MUMmer3.23