Report for Auto_user_IONAS-341-PHlab_170330_PHR19r-24r_443

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]8,147.51
‣ Number of Q20 Bases [Mbp]7,003.19
Total Number of Reads60,178,499
Mean Length [bp]135
Longest Read [bp]402

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_189%
TF_C94%

Test Fragment - TF_1

Quality Metrics

TF NameTF_1
TF SeqGAATAATCCAGCCCGCCAGGCATGGAAGAGCGTCGTAAAGTATTGCAGGT TCAGGCGGCGGAAAGCGTGATTGACTACTGGCAAATAAAGTACGTTCCAC CTTTGACACCATTTTCCGTAGTGAACTGACGCTGCCAAACGCCGACCGCG
Num264,790
Avg Q17 read length131
50AQ17236,653

Graphs

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num530,745
Avg Q17 read length90
50AQ17498,588

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 109,830,539 74%
Live ISPs100,235,483 91%
Test Fragment ISPs866,942 <1%
Library ISPs99,368,541 99%
CountPercentage
Library ISPs / Percent Enrichment99,368,541 91%
Filtered: Polyclonal28,539,655 29%
Filtered: Primer dimer260,109 <1%
Filtered: Low quality10,924,039 11%
Final Library Reads60,182,434 61%

Report Information

Analysis Info

Run Name R_2017_03_31_10_40_22_user_IONAS-341-PHlab_170330_PHR19r-24r
Run Date 2017-03-31 07:42:16+00:00
Run Flows 520
Project PHLab_RNASeq
Sample PHR21r-bcatMono
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes PHlab_6RNAs_PHR19r-24r_bc1-6
Barcode Set IonXpressRNA
Analysis Name Auto_user_IONAS-341-PHlab_170330_PHR19r-24r_443
Analysis Date 2017-03-31
runID KGZ3M

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1