Report for Auto_user_IONAS-338-PHlab_170310_PH3R8-22_438

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]9,942.63
‣ Number of Q20 Bases [Mbp]7,688.54
Total Number of Reads65,401,411
Mean Length [bp]152
Longest Read [bp]388

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]97.9976.19
Mean Length [bp]4537
Longest Alignment [bp]328312
Mean Coverage Depth0.04×0.03×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_189%
TF_C91%

Test Fragment - TF_1

Quality Metrics

TF NameTF_1
TF SeqGAATAATCCAGCCCGCCAGGCATGGAAGAGCGTCGTAAAGTATTGCAGGT TCAGGCGGCGGAAAGCGTGATTGACTACTGGCAAATAAAGTACGTTCCAC CTTTGACACCATTTTCCGTAGTGAACTGACGCTGCCAAACGCCGACCGCG
Num39,048
Avg Q17 read length131
50AQ1734,907

Graphs

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num189,548
Avg Q17 read length87
50AQ17173,404

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 132,824,593 90%
Live ISPs132,310,316 100%
Test Fragment ISPs243,620 <1%
Library ISPs132,066,696 100%
CountPercentage
Library ISPs / Percent Enrichment132,066,696 100%
Filtered: Polyclonal43,347,335 33%
Filtered: Primer dimer52,419 <1%
Filtered: Low quality24,297,542 18%
Final Library Reads65,716,117 50%

Report Information

Analysis Info

Run Name R_2017_03_10_12_21_37_user_IONAS-338-PHlab_170310_PH3R8-22
Run Date 2017-03-10 10:27:15+00:00
Run Flows 520
Project PHLab_3RNAseq,LexoGen_QuantSeq
Sample PH3R9-Con2
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes PHlab_AG-3RNA-6samples_PH3R8-14_VZ-3RNA-9samples_PH3R15-22
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-338-PHlab_170310_PH3R8-22_438
Analysis Date 2017-03-10
runID 6MVAE

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1