Report for Auto_user_ION-175-PHLab_150428_ChIRP-PHC47-50_240

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]3,433.60
‣ Number of Q20 Bases [Mbp]2,703.75
Total Number of Reads28,777,116
Mean Length [bp]119
Longest Read [bp]402

Reference Genome Information

Genome NameHomo sapiens
Genome Size3095693981
Genome Versionhg19
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]1,844.571,502.971,191.94
Mean Length [bp]1109881
Longest Alignment [bp]306279261
Mean Coverage Depth0.60×0.49×0.39×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C67%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num2,978,683
Avg Q17 read length62
50AQ171,982,206

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

block_X0_Y9324block_X1288_Y9324block_X2576_Y9324block_X3864_Y9324block_X5152_Y9324block_X6440_Y9324block_X7728_Y9324block_X9016_Y9324block_X10304_Y9324block_X11592_Y9324block_X12880_Y9324block_X14168_Y9324
block_X0_Y7992block_X1288_Y7992block_X2576_Y7992block_X3864_Y7992block_X5152_Y7992block_X6440_Y7992block_X7728_Y7992block_X9016_Y7992block_X10304_Y7992block_X11592_Y7992block_X12880_Y7992block_X14168_Y7992
block_X0_Y6660block_X1288_Y6660block_X2576_Y6660block_X3864_Y6660block_X5152_Y6660block_X6440_Y6660block_X7728_Y6660block_X9016_Y6660block_X10304_Y6660block_X11592_Y6660block_X12880_Y6660block_X14168_Y6660
block_X0_Y5328block_X1288_Y5328block_X2576_Y5328block_X3864_Y5328block_X5152_Y5328block_X6440_Y5328block_X7728_Y5328block_X9016_Y5328block_X10304_Y5328block_X11592_Y5328block_X12880_Y5328block_X14168_Y5328
block_X0_Y3996block_X1288_Y3996block_X2576_Y3996block_X3864_Y3996block_X5152_Y3996block_X6440_Y3996block_X7728_Y3996block_X9016_Y3996block_X10304_Y3996block_X11592_Y3996block_X12880_Y3996block_X14168_Y3996
block_X0_Y2664block_X1288_Y2664block_X2576_Y2664block_X3864_Y2664block_X5152_Y2664block_X6440_Y2664block_X7728_Y2664block_X9016_Y2664block_X10304_Y2664block_X11592_Y2664block_X12880_Y2664block_X14168_Y2664
block_X0_Y1332block_X1288_Y1332block_X2576_Y1332block_X3864_Y1332block_X5152_Y1332block_X6440_Y1332block_X7728_Y1332block_X9016_Y1332block_X10304_Y1332block_X11592_Y1332block_X12880_Y1332block_X14168_Y1332
block_X0_Y0block_X1288_Y0block_X2576_Y0block_X3864_Y0block_X5152_Y0block_X6440_Y0block_X7728_Y0block_X9016_Y0block_X10304_Y0block_X11592_Y0block_X12880_Y0block_X14168_Y0
CountPercentage
Total Addressable Wells148,603,956
‣ Wells with ISPs 119,569,027 80%
‣ Live ISPs119,414,964 100%
‣ Test Fragment ISPs3,204,056 3%
‣ Library ISPs116,210,908 97%
CountPercentage
Library ISPs / Percent Enrichment116,210,908 100%
‣ Filtered: Polyclonal45,554,255 39%
‣ Filtered: Primer dimer21,571,744 19%
‣ Filtered: Low quality22,312,376 19%
‣ Final Library Reads28,777,116 25%

Report Information

Analysis Info

Run Name R_2015_04_28_12_10_54_user_ION-175-PHLab_150428_ChIRP-PHC47-50
Run Date 2015-04-28 09:12:48+00:00
Run Flows 520
Project PHLab_ChIRP
Sample PHC49-Even
Library N/A
Reference hg19
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes PHlab_AG-4ChIRPsamples_PHC47-Input-bc4_PHC48-Odd-bc5_PHC49-Even-bc6_PHC50-LacZ-bc3
Barcode Set IonXpress
Analysis Name Auto_user_ION-175-PHLab_150428_ChIRP-PHC47-50_240
Analysis Date 2015-04-28
runID 6WA80

Software Version

Torrent_Suite4.4.2
hostFW7DQV1
ion-analysis4.4.8-1
ion-chefupdates4.4.5
ion-dbreports4.4.29-1
ion-gpu4.4.1-1
ion-pipeline4.4.11-1
ion-plugins4.4.14-1
ion-protonupdates4.4.3
ion-torrentr4.4.0-1
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