Report for user_ION-164-IT-miRNA_reanalysis_minlength

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]7,132.77
‣ Number of Q20 Bases [Mbp]5,797.19
Total Number of Reads75,502,283
Mean Length [bp]94
Longest Read [bp]378

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]4,011.863,395.072,766.57
Mean Length [bp]11710689
Longest Alignment [bp]304302293
Mean Coverage Depth1.51×1.28×1.04×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C76%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,939,348
Avg Q17 read length71
50AQ171,464,924

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,603,956
Wells with ISPs 130,200,220 88%
Live ISPs130,023,983 100%
Test Fragment ISPs2,234,573 2%
Library ISPs127,789,410 98%
CountPercentage
Library ISPs / Percent Enrichment127,789,410 100%
Filtered: Polyclonal45,157,339 35%
Filtered: Primer dimer261,944 <1%
Filtered: Low quality8,834,325 7%
Final Library Reads75,502,283 59%

Report Information

Analysis Info

Run Name R_2015_03_24_11_44_08_user_ION-164-IT-GKlab_150324_ChIP-miRNAtest_ITC46-7_miRNA1-6
Run Date 2015-03-24 09:46:06+00:00
Run Flows 520
Project ITLab_ChipSeq,GKLab_miRNAseq
Sample ITC47-Set8KO2input
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_10-ITsamples_ITC46-Set8KO1input-bc4_ITC47-Set8KO2input-bc6_GKlab_FG6miRNA-testsamples_3DifferentExtractionProtocols
Barcode Set IonXpress
Analysis Name user_ION-164-IT-miRNA_reanalysis_minlength
Analysis Date 2015-04-20
runID UKHW2

Software Version

Torrent_Suite4.4.2
hostFW7DQV1
ion-analysis4.4.8-1
ion-chefupdates4.4.5
ion-dbreports4.4.29-1
ion-gpu4.4.1-1
ion-pipeline4.4.11-1
ion-plugins4.4.14-1
ion-protonupdates4.4.3
ion-torrentr4.4.0-1