Report for Auto_user_ION-137-PHLab_150204_ChIRP-PHC41-46_202

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]8,403.43
‣ Number of Q20 Bases [Mbp]6,851.36
Total Number of Reads68,294,742
Mean Length [bp]123
Longest Read [bp]381

Reference Genome Information

Genome NameHomo sapiens
Genome Size3095693981
Genome Versionhg19
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]6,632.165,667.864,595.07
Mean Length [bp]11710689
Longest Alignment [bp]307289283
Mean Coverage Depth2.14×1.83×1.48×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C45%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num720,358
Avg Q17 read length44
50AQ17326,620

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,603,956
Wells with ISPs 124,042,358 83%
Live ISPs123,448,609 100%
Test Fragment ISPs883,244 <1%
Library ISPs122,565,365 99%
CountPercentage
Library ISPs / Percent Enrichment122,565,365 100%
Filtered: Polyclonal40,253,668 33%
Filtered: Primer dimer6,306,367 5%
Filtered: Low quality9,390,781 8%
Final Library Reads68,294,742 56%

Report Information

Analysis Info

Run Name R_2015_02_04_12_32_35_user_ION-137-PHLab_150204_ChIRP-PHC41-46
Run Date 2015-02-04 10:34:37+00:00
Run Flows 520
Project PHLab_ChIRP
Sample PHC42-EVEN1
Library N/A
Reference hg19
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes PHlab_AG-6ChIRP_PHC41-ODD1-bc8_PHC42-EVEN1-bc10_PHC43-LacZ1-bc11_PHC44-ODD2-bc12_PHC45-EVEN2-bc13_PHC46-LacZ2-bc16
Barcode Set IonXpress
Analysis Name Auto_user_ION-137-PHLab_150204_ChIRP-PHC41-46_202
Analysis Date 2015-02-04
runID H4PGZ

Software Version

Torrent_Suite4.2.1
Datacollect 2929
Graphics 34
LiveView 1835
OIA 4201
OS 21
hostFW7DQV1
ion-analysis4.2.18-1
ion-chefupdates4.2.0
ion-dbreports4.2.22-1
ion-gpu4.2.2-1
ion-pipeline4.2.12-1
ion-plugins4.2.28-1
ion-protonupdates4.2.2
ion-torrentr4.2.1-1