Dear George, the processing of the recent 2 samples has finished, results are in the folder: http://genomics-lab.fleming.gr/fleming/external/Sketas/run454 The alignments are in the bam files: GSkP7_A.bam 9235460 (48.65%) aligned reads GSkP8_b.bam 31433915 (73.96%) aligned reads The extracted insert sequences are in: GSkP7_A.fa GSkP8_b.fa The table combining the number of reads in each sample for each insert is in insert_samples_sorted.csv . As 2798911 different inserts are in this table, this cannot be viewed in Excel. A subset containing only inserts that have at least a total of 2 reads in both samples (837158 inserts) is in the table insert_samples_sorted_at_least_2_reads_total.xlsx Please let me know if you need additional information. Looking forward to the downstream results, best wishes, Martin