Report for Auto_user_ION-149-ITLab_DrMatis_150220_ITF4r_IMP1-3_214

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]10,983.27
‣ Number of Q20 Bases [Mbp]8,545.51
Total Number of Reads75,133,638
Mean Length [bp]146
Longest Read [bp]396

Reference Genome Information

Genome NameMus musculus
Genome Size2654911517
Genome Versionmm9
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]2,612.242,161.181,716.42
Mean Length [bp]11910688
Longest Alignment [bp]331319306
Mean Coverage Depth0.98×0.81×0.65×
Percentage of Library Covered N/A% N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C50%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,003,303
Avg Q17 read length49
50AQ17501,597

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,603,956
Wells with ISPs 136,882,139 92%
Live ISPs136,120,965 99%
Test Fragment ISPs1,409,998 1%
Library ISPs134,710,967 99%
CountPercentage
Library ISPs / Percent Enrichment134,710,967 99%
Filtered: Polyclonal52,163,400 39%
Filtered: Primer dimer106,349 <1%
Filtered: Low quality8,334,410 6%
Final Library Reads75,133,638 56%

Report Information

Analysis Info

Run Name R_2015_02_20_12_55_13_user_ION-149-ITLab_DrMatis_150220_ITF4r_IMP1-3_
Run Date 2015-02-20 10:58:05+00:00
Run Flows 520
Project ITLab_FaireSeq,DrMatis_DNA
Sample ITF4r-InputHNFaKO1
Library N/A
Reference mm9
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_YK12FaireSamples_ITF4r-InputHNFaKO1-bc6_DrMatis_3samples_IMP1_4-5-6-7PeptideLinrary-bc9_IMP2_A4VRound4-bc11_IMP3_Ab42Round2-bc15
Barcode Set IonXpress
Analysis Name Auto_user_ION-149-ITLab_DrMatis_150220_ITF4r_IMP1-3_214
Analysis Date 2015-02-20
runID ETB1X

Software Version

Torrent_Suite4.4
hostFW7DQV1
ion-analysis4.4.6-1
ion-chefupdates4.4.3
ion-dbreports4.4.22-1
ion-gpu4.4.1-1
ion-pipeline4.4.9-1
ion-plugins4.4.12-1
ion-protonupdates4.4.3
ion-torrentr4.4.0-1