Report for Auto_user_IONAS-369-ITlab_170906_ChIP_ITC298-301_473

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]399.78
‣ Number of Q20 Bases [Mbp]237.15
Total Number of Reads5,142,165
Mean Length [bp]77
Longest Read [bp]403

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C0%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num834,500
Avg Q17 read length12
50AQ17343

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 98,299,153 66%
Live ISPs87,580,358 89%
Test Fragment ISPs2,019,272 2%
Library ISPs85,561,086 98%
CountPercentage
Library ISPs / Percent Enrichment85,561,086 89%
Filtered: Polyclonal56,360,280 66%
Filtered: Primer dimer750,352 <1%
Filtered: Low quality24,507,354 29%
Final Library Reads5,143,219 6%

Report Information

Analysis Info

Run Name R_2017_09_07_15_12_04_user_IONAS-369-ITlab_170906_ChIP_ITC298-301
Run Date 2017-09-07 12:14:34+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC298-PolII-Untrwt3
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-Tereza_8ChIPseqs_PhosphoSeryn2POLII_Evaggelia_8ChIPseqs_POLII_PartRerunSeq170726
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-369-ITlab_170906_ChIP_ITC298-301_473
Analysis Date 2017-09-07
runID CKA4W

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1