Report for Auto_user_IONAS-368-ITlab_170906_ChIP_ITC302-305_474

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]10,537.55
‣ Number of Q20 Bases [Mbp]8,515.59
Total Number of Reads74,734,649
Mean Length [bp]140
Longest Read [bp]380

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C77%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,242,661
Avg Q17 read length73
50AQ17956,468

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 129,553,318 87%
Live ISPs129,537,017 100%
Test Fragment ISPs1,340,385 1%
Library ISPs128,196,632 99%
CountPercentage
Library ISPs / Percent Enrichment128,196,632 100%
Filtered: Polyclonal35,637,382 28%
Filtered: Primer dimer1,947,346 2%
Filtered: Low quality18,407,147 14%
Final Library Reads74,769,225 58%

Report Information

Analysis Info

Run Name R_2017_09_07_12_31_58_user_IONAS-368-ITlab_170906_ChIP_ITC302-305
Run Date 2017-09-07 09:34:24+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC303-PolII-DENTCPwt3
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-Tereza_8ChIPseqs_PhosphoSeryn2POLII_Evaggelia_8ChIPseqs_POLII_PartRerunSeq170726
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-368-ITlab_170906_ChIP_ITC302-305_474
Analysis Date 2017-09-07
runID QPKJS

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1