Report for Auto_user_IONAS-367-ITlab_170905_ChIP_ITC290-293-296-7_471

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]10,660.30
‣ Number of Q20 Bases [Mbp]8,637.27
Total Number of Reads74,278,404
Mean Length [bp]143
Longest Read [bp]387

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C86%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,586,448
Avg Q17 read length81
50AQ171,358,402

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 121,484,934 82%
Live ISPs120,927,510 100%
Test Fragment ISPs1,817,255 2%
Library ISPs119,110,255 98%
CountPercentage
Library ISPs / Percent Enrichment119,110,255 100%
Filtered: Polyclonal35,011,857 29%
Filtered: Primer dimer2,456,626 2%
Filtered: Low quality9,334,312 8%
Final Library Reads74,422,790 62%

Report Information

Analysis Info

Run Name R_2017_09_06_15_17_51_user_IONAS-367-ITlab_170905_ChIP_ITC290-293-296-7
Run Date 2017-09-06 12:19:58+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC296-Pho-DENAlSm3
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-Tereza_8ChIPseqs_PhosphoSeryn2POLII_Evaggelia_8ChIPseqs_POLII_PartRerunSeq170726
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-367-ITlab_170905_ChIP_ITC290-293-296-7_471
Analysis Date 2017-09-06
runID WWNFK

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1