Report for Auto_user_IONAS-366-ITlab_170905_ChIP_ITC290-293-5_472

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]10,960.60
‣ Number of Q20 Bases [Mbp]8,985.07
Total Number of Reads76,126,443
Mean Length [bp]143
Longest Read [bp]391

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C85%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,679,011
Avg Q17 read length80
50AQ171,425,990

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 125,779,571 85%
Live ISPs125,519,372 100%
Test Fragment ISPs1,972,401 2%
Library ISPs123,546,971 98%
CountPercentage
Library ISPs / Percent Enrichment123,546,971 100%
Filtered: Polyclonal37,529,295 30%
Filtered: Primer dimer3,114,463 3%
Filtered: Low quality8,864,155 7%
Final Library Reads76,357,135 62%

Report Information

Analysis Info

Run Name R_2017_09_06_12_28_41_user_IONAS-366-ITlab_170905_ChIP_ITC290-293-5
Run Date 2017-09-06 09:30:25+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC295-Pho-AlSm2
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-Tereza_8ChIPseqs_PhosphoSeryn2POLII_Evaggelia_8ChIPseqs_POLII_PartRerunSeq170726
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-366-ITlab_170905_ChIP_ITC290-293-5_472
Analysis Date 2017-09-06
runID WOTZH

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1