Report for Auto_user_IONAS-365-ITlab_170904_ChIP_ITC274-277_469

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]11,483.91
‣ Number of Q20 Bases [Mbp]9,014.85
Total Number of Reads77,483,073
Mean Length [bp]148
Longest Read [bp]388

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C78%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num934,974
Avg Q17 read length74
50AQ17729,170

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 132,746,737 90%
Live ISPs132,482,887 100%
Test Fragment ISPs1,165,103 <1%
Library ISPs131,317,784 99%
CountPercentage
Library ISPs / Percent Enrichment131,317,784 100%
Filtered: Polyclonal45,074,004 34%
Filtered: Primer dimer720,147 <1%
Filtered: Low quality10,317,912 8%
Final Library Reads77,485,824 59%

Report Information

Analysis Info

Run Name R_2017_09_05_14_47_34_user_IONAS-365-ITlab_170904_ChIP_ITC274-277
Run Date 2017-09-05 11:49:52+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC274-PolII-wt3
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-Tereza_8ChIPseqs_POLII_PartRerunSeq170726
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-365-ITlab_170904_ChIP_ITC274-277_469
Analysis Date 2017-09-05
runID JTAPY

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1