Report for Auto_user_IONAS-364-ITlab_170904_ChIP_ITC278-281_274_470

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]4,892.60
‣ Number of Q20 Bases [Mbp]3,854.76
Total Number of Reads34,073,271
Mean Length [bp]143
Longest Read [bp]386

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C56%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num333,450
Avg Q17 read length53
50AQ17185,362

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 90,972,751 61%
Live ISPs67,634,688 74%
Test Fragment ISPs536,465 <1%
Library ISPs67,098,223 99%
CountPercentage
Library ISPs / Percent Enrichment67,098,223 74%
Filtered: Polyclonal22,983,594 34%
Filtered: Primer dimer317,659 <1%
Filtered: Low quality11,105,131 17%
Final Library Reads34,078,096 51%

Report Information

Analysis Info

Run Name R_2017_09_05_12_00_46_user_IONAS-364-ITlab_170904_ChIP_ITC278-281_274
Run Date 2017-09-05 09:02:43+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC278-PolII-AlSm1
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-Tereza_8ChIPseqs_POLII_PartRerunSeq170726_ITC274-TechnicalReplicate
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-364-ITlab_170904_ChIP_ITC278-281_274_470
Analysis Date 2017-09-05
runID XK56V

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1