Report for Auto_user_IONAS-358-ITlab_170726_ChIP_ITC290-305_463

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]9,629.06
‣ Number of Q20 Bases [Mbp]7,572.65
Total Number of Reads68,678,809
Mean Length [bp]140
Longest Read [bp]395

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C81%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num943,579
Avg Q17 read length77
50AQ17762,400

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 124,961,142 84%
Live ISPs124,654,640 100%
Test Fragment ISPs1,092,039 <1%
Library ISPs123,562,601 99%
CountPercentage
Library ISPs / Percent Enrichment123,562,601 100%
Filtered: Polyclonal39,835,226 32%
Filtered: Primer dimer2,316,158 2%
Filtered: Low quality15,084,880 12%
Final Library Reads68,755,641 56%

Report Information

Analysis Info

Run Name R_2017_07_27_14_55_50_user_IONAS-358-ITlab_170726_ChIP_ITC290-305
Run Date 2017-07-27 11:57:52+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC298-PolII-Untrwt3
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-Tereza_8ChIPseqs_PhosphoSeryn2POLII_Evaggelia_8ChIPseqs_POLII
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-358-ITlab_170726_ChIP_ITC290-305_463
Analysis Date 2017-07-27
runID CJ591

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1