Report for Auto_user_IONAS-357-ITlab_170726_ChIP_ITC274-289_462

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]10,359.74
‣ Number of Q20 Bases [Mbp]7,902.83
Total Number of Reads71,497,999
Mean Length [bp]144
Longest Read [bp]386

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C70%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num657,421
Avg Q17 read length67
50AQ17463,392

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 132,167,717 89%
Live ISPs131,877,846 100%
Test Fragment ISPs859,166 <1%
Library ISPs131,018,680 99%
CountPercentage
Library ISPs / Percent Enrichment131,018,680 100%
Filtered: Polyclonal47,842,072 37%
Filtered: Primer dimer1,285,591 <1%
Filtered: Low quality12,987,249 10%
Final Library Reads71,503,433 55%

Report Information

Analysis Info

Run Name R_2017_07_27_12_04_09_user_IONAS-357-ITlab_170726_ChIP_ITC274-289_
Run Date 2017-07-27 09:06:26+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC280-PolII-DENAlSm3
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-Tereza_16ChIPseqs_POLII-H2BmonomethylK5
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-357-ITlab_170726_ChIP_ITC274-289_462
Analysis Date 2017-07-27
runID CYA3N

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1