Report for Auto_user_IONAS-355-ITLab_170726_ITRC27-41_458

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]7,393.76
‣ Number of Q20 Bases [Mbp]5,790.81
Total Number of Reads51,620,780
Mean Length [bp]143
Longest Read [bp]401

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C94%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,771,301
Avg Q17 read length90
50AQ171,668,442

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 104,661,149 71%
Live ISPs104,427,497 100%
Test Fragment ISPs1,836,838 2%
Library ISPs102,590,659 98%
CountPercentage
Library ISPs / Percent Enrichment102,590,659 100%
Filtered: Polyclonal20,076,378 20%
Filtered: Primer dimer433,623 <1%
Filtered: Low quality32,287,673 31%
Final Library Reads51,620,785 50%

Report Information

Analysis Info

Run Name R_2017_07_26_13_31_42_user_IONAS-355-ITLab_170726_ITRC27-41
Run Date 2017-07-26 10:33:38+00:00
Run Flows 520
Project ITLab_uRNA
Sample ITRC38-EU-DENTC-wt2
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_15uRNAs_ITRC27-41
Barcode Set IonXpressRNA
Analysis Name Auto_user_IONAS-355-ITLab_170726_ITRC27-41_458
Analysis Date 2017-07-26
runID 7WZYV

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1