Report for Auto_user_IONAS-350-ITlab_170608_ChIP_ITC265-269_453

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]10,413.22
‣ Number of Q20 Bases [Mbp]9,112.42
Total Number of Reads75,524,005
Mean Length [bp]137
Longest Read [bp]380

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]2,787.352,301.02
Mean Length [bp]119103
Longest Alignment [bp]334320
Mean Coverage Depth1.02×0.84×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_191%
TF_C95%

Test Fragment - TF_1

Quality Metrics

TF NameTF_1
TF SeqGAATAATCCAGCCCGCCAGGCATGGAAGAGCGTCGTAAAGTATTGCAGGT TCAGGCGGCGGAAAGCGTGATTGACTACTGGCAAATAAAGTACGTTCCAC CTTTGACACCATTTTCCGTAGTGAACTGACGCTGCCAAACGCCGACCGCG
Num465,711
Avg Q17 read length133
50AQ17424,608

Graphs

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num715,822
Avg Q17 read length90
50AQ17676,475

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 133,567,631 90%
Live ISPs133,509,482 100%
Test Fragment ISPs1,240,075 <1%
Library ISPs132,269,407 99%
CountPercentage
Library ISPs / Percent Enrichment132,269,407 100%
Filtered: Polyclonal37,447,334 28%
Filtered: Primer dimer3,412,768 3%
Filtered: Low quality16,237,253 12%
Final Library Reads75,894,102 57%

Report Information

Analysis Info

Run Name R_2017_06_09_13_18_10_user_IONAS-350-ITlab_170608_ChIP_ITC265-269
Run Date 2017-06-09 10:20:06+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC268-Mar5
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_EP11samples_ITC265-267_koDENTCP_6MarousoSamples_ITC268-9
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-350-ITlab_170608_ChIP_ITC265-269_453
Analysis Date 2017-06-09
runID DKFLP

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1