Report for Auto_user_IONAS-345-ITLab_170515_ITR51r-52_ITRC25_448

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]9,322.77
‣ Number of Q20 Bases [Mbp]7,491.04
Total Number of Reads90,051,474
Mean Length [bp]103
Longest Read [bp]394

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ17AQ20Perfect
Total Number of Bases [Mbp]0.000.000.00
Mean Length [bp]000
Longest Alignment [bp]000

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_190%
TF_C93%

Test Fragment - TF_1

Quality Metrics

TF NameTF_1
TF SeqGAATAATCCAGCCCGCCAGGCATGGAAGAGCGTCGTAAAGTATTGCAGGT TCAGGCGGCGGAAAGCGTGATTGACTACTGGCAAATAAAGTACGTTCCAC CTTTGACACCATTTTCCGTAGTGAACTGACGCTGCCAAACGCCGACCGCG
Num410,302
Avg Q17 read length129
50AQ17368,849

Graphs

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num292,046
Avg Q17 read length89
50AQ17271,344

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

block_X0_Y9324block_X1288_Y9324block_X2576_Y9324block_X3864_Y9324block_X5152_Y9324block_X6440_Y9324block_X7728_Y9324block_X9016_Y9324block_X10304_Y9324block_X11592_Y9324block_X12880_Y9324block_X14168_Y9324
block_X0_Y7992block_X1288_Y7992block_X2576_Y7992block_X3864_Y7992block_X5152_Y7992block_X6440_Y7992block_X7728_Y7992block_X9016_Y7992block_X10304_Y7992block_X11592_Y7992block_X12880_Y7992block_X14168_Y7992
block_X0_Y6660block_X1288_Y6660block_X2576_Y6660block_X3864_Y6660block_X5152_Y6660block_X6440_Y6660block_X7728_Y6660block_X9016_Y6660block_X10304_Y6660block_X11592_Y6660block_X12880_Y6660block_X14168_Y6660
block_X0_Y5328block_X1288_Y5328block_X2576_Y5328block_X3864_Y5328block_X5152_Y5328block_X6440_Y5328block_X7728_Y5328block_X9016_Y5328block_X10304_Y5328block_X11592_Y5328block_X12880_Y5328block_X14168_Y5328
block_X0_Y3996block_X1288_Y3996block_X2576_Y3996block_X3864_Y3996block_X5152_Y3996block_X6440_Y3996block_X7728_Y3996block_X9016_Y3996block_X10304_Y3996block_X11592_Y3996block_X12880_Y3996block_X14168_Y3996
block_X0_Y2664block_X1288_Y2664block_X2576_Y2664block_X3864_Y2664block_X5152_Y2664block_X6440_Y2664block_X7728_Y2664block_X9016_Y2664block_X10304_Y2664block_X11592_Y2664block_X12880_Y2664block_X14168_Y2664
block_X0_Y1332block_X1288_Y1332block_X2576_Y1332block_X3864_Y1332block_X5152_Y1332block_X6440_Y1332block_X7728_Y1332block_X9016_Y1332block_X10304_Y1332block_X11592_Y1332block_X12880_Y1332block_X14168_Y1332
block_X0_Y0block_X1288_Y0block_X2576_Y0block_X3864_Y0block_X5152_Y0block_X6440_Y0block_X7728_Y0block_X9016_Y0block_X10304_Y0block_X11592_Y0block_X12880_Y0block_X14168_Y0
CountPercentage
Total Addressable Wells148,155,732
‣ Wells with ISPs 134,556,806 91%
‣ Live ISPs134,516,687 100%
‣ Test Fragment ISPs737,911 <1%
‣ Library ISPs133,778,776 99%
CountPercentage
Library ISPs / Percent Enrichment133,778,776 100%
‣ Filtered: Polyclonal32,986,767 25%
‣ Filtered: Primer dimer222,111 <1%
‣ Filtered: Low quality11,489,253 9%
‣ Final Library Reads90,054,115 67%

Report Information

Analysis Info

Run Name R_2017_05_16_12_13_35_user_IONAS-345-ITLab_170515_ITR51r-52_ITRC25
Run Date 2017-05-16 09:15:26+00:00
Run Flows 520
Project ITLab_uRNA,ITLab_RNASeq
Sample ITR51r-Set9ko1
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_7RNAseqSamples_ITRC2EPsamples
Barcode Set IonXpressRNA
Analysis Name Auto_user_IONAS-345-ITLab_170515_ITR51r-52_ITRC25_448
Analysis Date 2017-05-16
runID 0Q5IL

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1
Request Support  |  Help/Documentation  |  Terms of Use
Copyright © 2012 Life Technologies Corporation
This product should be used for research use only