Report for Auto_user_IONAS-336-ITlab_170216_ChIP_ITC243-246_436

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]6,518.44
‣ Number of Q20 Bases [Mbp]5,259.01
Total Number of Reads51,431,029
Mean Length [bp]126
Longest Read [bp]396

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]4,045.913,199.37
Mean Length [bp]10990
Longest Alignment [bp]318306
Mean Coverage Depth1.48×1.17×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C83%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,425,774
Avg Q17 read length79
50AQ171,180,866

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 117,521,832 79%
Live ISPs116,567,794 99%
Test Fragment ISPs1,587,043 1%
Library ISPs114,980,751 99%
CountPercentage
Library ISPs / Percent Enrichment114,980,751 99%
Filtered: Polyclonal43,125,748 38%
Filtered: Primer dimer1,105,401 <1%
Filtered: Low quality19,992,653 17%
Final Library Reads51,432,979 45%

Report Information

Analysis Info

Run Name R_2017_02_16_12_09_42_user_IONAS-336-ITlab_170216_ChIP_ITC243-246
Run Date 2017-02-16 10:11:12+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC245-wt1DENTCP-Usp
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_EP28samples_ITC243-246_wt-wtDENTCP-Usp22origene
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-336-ITlab_170216_ChIP_ITC243-246_436
Analysis Date 2017-02-16
runID 494AS

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1