Report for Auto_user_IONAS-332-ITlab_170213_ITRC17-23_433

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]2,558.61
‣ Number of Q20 Bases [Mbp]2,164.21
Total Number of Reads66,365,475
Mean Length [bp]38
Longest Read [bp]399

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C94%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num2,026,345
Avg Q17 read length89
50AQ171,904,697

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 115,933,792 78%
Live ISPs115,922,931 100%
Test Fragment ISPs2,121,727 2%
Library ISPs113,801,204 98%
CountPercentage
Library ISPs / Percent Enrichment113,801,204 100%
Filtered: Polyclonal23,691,495 21%
Filtered: Primer dimer4,588,419 4%
Filtered: Low quality20,085,837 18%
Final Library Reads66,370,018 58%

Report Information

Analysis Info

Run Name R_2017_02_14_11_22_48_user_IONAS-332-ITlab_170213_ITRC17-23
Run Date 2017-02-14 09:24:47+00:00
Run Flows 520
Project ITLab_uRNA
Sample ITRC18-wt2Br
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_6uRNAs_wtvsko_3Br_3Eu
Barcode Set IonXpressRNA
Analysis Name Auto_user_IONAS-332-ITlab_170213_ITRC17-23_433
Analysis Date 2017-02-14
runID A00QW

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1