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Report for Auto_user_IONAS-331-ITlab_170209_ChIP_ITC248-251_430

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]11,124.75
‣ Number of Q20 Bases [Mbp]9,399.14
Total Number of Reads78,455,887
Mean Length [bp]141
Longest Read [bp]386

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]7,914.246,315.11
Mean Length [bp]124103
Longest Alignment [bp]342332
Mean Coverage Depth2.90×2.32×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C93%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,112,510
Avg Q17 read length88
50AQ171,030,014

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 133,602,920 90%
Live ISPs133,333,663 100%
Test Fragment ISPs1,250,282 <1%
Library ISPs132,083,381 99%
CountPercentage
Library ISPs / Percent Enrichment132,083,381 100%
Filtered: Polyclonal45,702,369 35%
Filtered: Primer dimer1,153,479 <1%
Filtered: Low quality7,417,497 6%
Final Library Reads78,480,998 59%

Report Information

Analysis Info

Run Name R_2017_02_10_14_00_13_user_IONAS-331-ITlab_170209_ChIP_ITC248-251
Run Date 2017-02-10 12:02:33+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC249-E151
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_YK9samples_ITC248-251_FOXA1
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-331-ITlab_170209_ChIP_ITC248-251_430
Analysis Date 2017-02-10
runID BBBB4

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1