Report for Auto_user_IONAS-329-ITlab_170119_IT3R49-58_428

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]4,573.94
‣ Number of Q20 Bases [Mbp]3,614.52
Total Number of Reads43,374,832
Mean Length [bp]105
Longest Read [bp]391

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]1,414.94869.21
Mean Length [bp]9372
Longest Alignment [bp]322297
Mean Coverage Depth0.52×0.32×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C85%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,540,631
Avg Q17 read length80
50AQ171,314,764

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 94,082,472 64%
Live ISPs93,723,938 100%
Test Fragment ISPs1,589,464 2%
Library ISPs92,134,474 98%
CountPercentage
Library ISPs / Percent Enrichment92,134,474 100%
Filtered: Polyclonal24,347,988 26%
Filtered: Primer dimer90,421 <1%
Filtered: Low quality24,253,743 26%
Final Library Reads43,904,396 48%

Report Information

Analysis Info

Run Name R_2017_01_20_13_47_13_user_IONAS-329-ITlab_170119_IT3R49-58
Run Date 2017-01-20 11:49:30+00:00
Run Flows 520
Project ITLab_3RNAseq,LexoGen_QuantSeq
Sample IT3R52-wt6
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_EP-3RNA-10samples_IT3R49-58_wtDENTCP-USP22koDENTCP
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-329-ITlab_170119_IT3R49-58_428
Analysis Date 2017-01-20
runID XPTYT

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1