#@ Dear Martin, we would also like to compare these new RNAseq data with the previous ones ''ITlab run299''. Specifically we want : a) IT3R23-26 (4 WT untreated samples) vs IT3R49-IT3R53 (5 WT DENTCP samples) b) IT3R27-30 (4 KO untreated samples) vs IT3R54-IT3R58 (5 KO DENTCP samples) Ξ’est Evaggelia #@ Dear Evanggelia, at http://genomics-lab.fleming.gr/cgi-bin/hgTracks?db=mm10&hubUrl=http://genomics-lab.fleming.gr/fleming/ITlab/run329/hub.txt you'll find tracks, normalized to the smallest library (IT3R58-ko7 with 1488048 reads). At http://genomics-lab.fleming.gr/fleming/ITlab/run329/metaseqr_quantseq_wt_vs_ko/index.html is the metaseqR analysis for the contrast wt vs ko (wt is the reference). Let me known if you need additional contrasts. Best wishes, Martin sample n_reads IT3R49-wt1 4292833 IT3R50-wt3 3700750 IT3R51-wt4 3705100 IT3R52-wt6 4740856 IT3R53-wt7 2599816 IT3R54-ko1 5836320 IT3R55-ko3 4893272 IT3R56-ko4 5230261 IT3R57-ko6 4290827 IT3R58-ko7 1488048