Report for Auto_user_IONAS-328-ITlab_170119_ITRC11b-14-15-16_429

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]5,055.69
‣ Number of Q20 Bases [Mbp]4,191.43
Total Number of Reads56,853,275
Mean Length [bp]88
Longest Read [bp]382

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C94%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,883,637
Avg Q17 read length89
50AQ171,765,366

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 112,619,286 76%
Live ISPs112,571,403 100%
Test Fragment ISPs1,969,774 2%
Library ISPs110,601,629 98%
CountPercentage
Library ISPs / Percent Enrichment110,601,629 100%
Filtered: Polyclonal27,053,421 24%
Filtered: Primer dimer14,112,025 13%
Filtered: Low quality13,208,604 12%
Final Library Reads56,857,583 51%

Report Information

Analysis Info

Run Name R_2017_01_20_11_06_39_user_IONAS-328-ITlab_170119_ITRC11b-14-15-16
Run Date 2017-01-20 09:08:59+00:00
Run Flows 520
Project ITLab_uRNA
Sample ITRC16-SET8ko2EUstrep
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_4uRNAs_wtvsko_EUStrept
Barcode Set IonXpressRNA
Analysis Name Auto_user_IONAS-328-ITlab_170119_ITRC11b-14-15-16_429
Analysis Date 2017-01-20
runID 3OO5Q

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1