Report for Auto_user_IONAS-327-ITlab_161228_IT3R35-48_427

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]7,846.34
‣ Number of Q20 Bases [Mbp]6,087.83
Total Number of Reads66,788,933
Mean Length [bp]117
Longest Read [bp]390

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]1,430.72846.35
Mean Length [bp]9674
Longest Alignment [bp]309284
Mean Coverage Depth0.52×0.31×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C68%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num2,451,810
Avg Q17 read length64
50AQ171,662,792

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 121,576,121 82%
Live ISPs120,187,739 99%
Test Fragment ISPs3,293,846 3%
Library ISPs116,893,893 97%
CountPercentage
Library ISPs / Percent Enrichment116,893,893 99%
Filtered: Polyclonal41,774,109 36%
Filtered: Primer dimer121,933 <1%
Filtered: Low quality8,093,876 7%
Final Library Reads66,914,794 57%

Report Information

Analysis Info

Run Name R_2016_12_28_12_39_18_user_IONAS-327-ITlab_161228_IT3R35-48
Run Date 2016-12-28 10:41:00+00:00
Run Flows 520
Project ITLab_3RNAseq,LexoGen_QuantSeq
Sample IT3R37-3343-2
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_RR-3RNA-14samples_IT3R35-48_bc33-46
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-327-ITlab_161228_IT3R35-48_427
Analysis Date 2016-12-28
runID 969QG

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1