Report for Auto_user_IONAS-324-IT-PHlab_161220_PH3R1-6_ITRC11-13_424

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]6,060.46
‣ Number of Q20 Bases [Mbp]4,249.92
Total Number of Reads60,245,487
Mean Length [bp]100
Longest Read [bp]401

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C87%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num4,648,526
Avg Q17 read length82
50AQ174,039,956

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 114,310,012 77%
Live ISPs113,095,289 99%
Test Fragment ISPs5,056,201 4%
Library ISPs108,039,088 96%
CountPercentage
Library ISPs / Percent Enrichment108,039,088 99%
Filtered: Polyclonal33,961,441 31%
Filtered: Primer dimer380,270 <1%
Filtered: Low quality13,468,540 12%
Final Library Reads60,254,248 56%

Report Information

Analysis Info

Run Name R_2016_12_20_11_18_53_user_IONAS-324-IT-PHlab_161220_PH3R1-6_ITRC11-13
Run Date 2016-12-20 09:20:25+00:00
Run Flows 520
Project ITLab_uRNA,PHLab_3RNAseq
Sample PH3R4-Ig3-1
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes PHLab-6-3RNAseq_ITLab-3of9-uRNAs
Barcode Set IonXpressRNA
Analysis Name Auto_user_IONAS-324-IT-PHlab_161220_PH3R1-6_ITRC11-13_424
Analysis Date 2016-12-20
runID 53RDI

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1