Report for Auto_user_IONAS-323-ITlab_161209_ChIP_ITC213-16-219_423

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]8,862.09
‣ Number of Q20 Bases [Mbp]7,116.38
Total Number of Reads72,876,569
Mean Length [bp]121
Longest Read [bp]393

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]4,093.213,287.92
Mean Length [bp]10286
Longest Alignment [bp]317293
Mean Coverage Depth1.50×1.21×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C73%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,812,587
Avg Q17 read length69
50AQ171,328,596

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 132,046,981 89%
Live ISPs131,305,996 99%
Test Fragment ISPs2,493,988 2%
Library ISPs128,812,008 98%
CountPercentage
Library ISPs / Percent Enrichment128,812,008 99%
Filtered: Polyclonal44,046,786 34%
Filtered: Primer dimer1,748,641 1%
Filtered: Low quality10,136,187 8%
Final Library Reads72,908,547 57%

Report Information

Analysis Info

Run Name R_2016_12_09_11_27_01_user_IONAS-323-ITlab_161209_ChIP_ITC213-16-219
Run Date 2016-12-09 09:28:38+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC213-wt1Me3
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab_SG-19samples_ITC213-wt1Me3-bc19_ITC214-wt1TCPMe3-bc20_ITC215-wt3Me1-bc21_ITC216-wt3TCPMe1-bc22_ITC219-SET8ko2TCPINPUT-bc25
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-323-ITlab_161209_ChIP_ITC213-16-219_423
Analysis Date 2016-12-09
runID 3FXW7

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1