Report for Auto_user_IONAS-314-ITlab_161110_CHEF_ChIP_ITC171-174_411

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]10,476.99
‣ Number of Q20 Bases [Mbp]9,103.05
Total Number of Reads71,541,122
Mean Length [bp]146
Longest Read [bp]389

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]7,009.865,673.09
Mean Length [bp]134113
Longest Alignment [bp]341336
Mean Coverage Depth2.57×2.08×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C90%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,454,175
Avg Q17 read length85
50AQ171,314,172

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 122,304,263 83%
Live ISPs121,613,070 99%
Test Fragment ISPs1,671,528 1%
Library ISPs119,941,542 99%
CountPercentage
Library ISPs / Percent Enrichment119,941,542 99%
Filtered: Polyclonal37,882,335 32%
Filtered: Primer dimer3,703,521 3%
Filtered: Low quality7,473,051 6%
Final Library Reads71,554,401 60%

Report Information

Analysis Info

Run Name R_2016_11_11_13_36_20_user_IONAS-314-ITlab_161110_CHEF_ChIP_ITC171-174
Run Date 2016-11-11 11:38:13+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC174-4
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-24EP-ChIPs_ITC171-1-bc1_ITC172-2-bc2_ITC173-3-bc3_ITC174-4-bc4
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-314-ITlab_161110_CHEF_ChIP_ITC171-174_411
Analysis Date 2016-11-11
runID U0RPC

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1