Report for Auto_user_IONAS-312-ITlab_161025_CHEF_ChIP_ITC167-170_410

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]3,737.45
‣ Number of Q20 Bases [Mbp]3,239.18
Total Number of Reads27,800,762
Mean Length [bp]134
Longest Read [bp]392

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]2,746.552,248.42
Mean Length [bp]120102
Longest Alignment [bp]325317
Mean Coverage Depth1.01×0.82×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C82%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num328,077
Avg Q17 read length78
50AQ17269,145

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 81,406,323 55%
Live ISPs52,635,241 65%
Test Fragment ISPs425,048 <1%
Library ISPs52,210,193 99%
CountPercentage
Library ISPs / Percent Enrichment52,210,193 64%
Filtered: Polyclonal14,049,693 27%
Filtered: Primer dimer441,745 <1%
Filtered: Low quality10,121,312 19%
Final Library Reads27,808,253 53%

Report Information

Analysis Info

Run Name R_2016_10_26_14_13_40_user_IONAS-312-ITlab_161025_CHEF_ChIP_ITC167-170
Run Date 2016-10-26 11:15:44+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC170-12a
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-24EP-ChIPs_ITC167-9a-bc13_ITC168-10a-bc14_ITC169-11a-bc15_ITC170-12a-bc16
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-312-ITlab_161025_CHEF_ChIP_ITC167-170_410
Analysis Date 2016-10-26
runID PRFFA

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1