Report for Auto_user_IONAS-310-ITlab_161018_CHEF_ChIP_ITC155-158_407

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]8,586.53
‣ Number of Q20 Bases [Mbp]7,408.89
Total Number of Reads64,229,830
Mean Length [bp]133
Longest Read [bp]373

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]5,025.504,095.09
Mean Length [bp]122104
Longest Alignment [bp]332323
Mean Coverage Depth1.84×1.50×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_190%
TF_C95%

Test Fragment - TF_1

Quality Metrics

TF NameTF_1
TF SeqGAATAATCCAGCCCGCCAGGCATGGAAGAGCGTCGTAAAGTATTGCAGGT TCAGGCGGCGGAAAGCGTGATTGACTACTGGCAAATAAAGTACGTTCCAC CTTTGACACCATTTTCCGTAGTGAACTGACGCTGCCAAACGCCGACCGCG
Num484,466
Avg Q17 read length131
50AQ17435,733

Graphs

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num427,920
Avg Q17 read length91
50AQ17407,202

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 130,030,111 88%
Live ISPs129,868,526 100%
Test Fragment ISPs996,759 <1%
Library ISPs128,871,767 99%
CountPercentage
Library ISPs / Percent Enrichment128,871,767 100%
Filtered: Polyclonal42,551,779 33%
Filtered: Primer dimer2,412,659 2%
Filtered: Low quality20,191,535 16%
Final Library Reads64,242,185 50%

Report Information

Analysis Info

Run Name R_2016_10_19_14_17_35_user_IONAS-310-ITlab_161018_CHEF_ChIP_ITC155-158
Run Date 2016-10-19 11:19:48+00:00
Run Flows 520
Project ITLab_ChipSeq
Sample ITC156-FOXAIS2
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITlab-4YK-ChIPs_ITC155-FOXAIS1-bc1_ITC156-FOXAIS2-bc2_ITC157-FOXAPO1-bc3_ITC158-FOXAPO2-bc4
Barcode Set IonXpress
Analysis Name Auto_user_IONAS-310-ITlab_161018_CHEF_ChIP_ITC155-158_407
Analysis Date 2016-10-19
runID FF3BU

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1