Report for Auto_user_IONAS-308-Pasteur_ITLab_161007_IPR23r-30_ITRC3-6_406

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]8,742.88
‣ Number of Q20 Bases [Mbp]6,601.67
Total Number of Reads87,683,330
Mean Length [bp]99
Longest Read [bp]365

Reference Genome Information

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]0.000.00
Mean Length [bp]00
Longest Alignment [bp]00

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C82%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num1,550,368
Avg Q17 read length77
50AQ171,272,935

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 134,610,415 91%
Live ISPs134,342,803 100%
Test Fragment ISPs1,796,525 1%
Library ISPs132,546,278 99%
CountPercentage
Library ISPs / Percent Enrichment132,546,278 100%
Filtered: Polyclonal38,760,977 29%
Filtered: Primer dimer1,798,124 1%
Filtered: Low quality4,874,502 4%
Final Library Reads87,814,604 66%

Report Information

Analysis Info

Run Name R_2016_10_07_11_09_49_user_IONAS-308-Pasteur_ITLab_161007_IPR23r-30_ITRC3-6
Run Date 2016-10-07 08:11:14+00:00
Run Flows 500
Project Pasteur_RNAseq,ITLab_uRNA
Sample ITRC3-wt2
Library N/A
Reference
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes Pasteur_MA-2amples_IPR23r-MEDC_IPR30-MEDB_ITlab_KN-4uRNAs-NRO_ITRC3-wt2_ITRC4-wt3_ITRC5-SET8ko2_ITRC6-SET8ko3
Barcode Set IonXpressRNA
Analysis Name Auto_user_IONAS-308-Pasteur_ITLab_161007_IPR23r-30_ITRC3-6_406
Analysis Date 2016-10-07
runID RTGS7

Software Version

Torrent_Suite5.0.5
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.7
ion-dbreports5.0.34-1
ion-gpu5.0.0-1
ion-pipeline5.0.17-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1