Report for IONAS-304-ITLab_MV-MuSeek_160920_ITMu1-2

Library Summary

Based on Predicted Per-Base Quality Scores - Independent of Alignment

Total Number of Bases [Mbp]4,579.91
‣ Number of Q20 Bases [Mbp]3,935.40
Total Number of Reads38,524,120
Mean Length [bp]118
Longest Read [bp]388

Reference Genome Information

Genome Namemm10
Genome Size2725537669
Genome Version1
Index Versiontmap-f3

Based on Full Library Alignment to Provided Reference

AQ20Perfect
Total Number of Bases [Mbp]190.65182.57
Mean Length [bp]4947
Longest Alignment [bp]265242
Mean Coverage Depth0.07×0.07×
Percentage of Library Covered N/A% N/A%

Test Fragment Report

Test Fragment Summary

Test FragmentPercent (50AQ17 / Num)
TF_C82%

Test Fragment - TF_C

Quality Metrics

TF NameTF_C
TF SeqTACGAGCGTGTAGACGTGTCGTACGTGCGACGTAGTGAGTATACATGCTC TGACACTATGTACGATCTGAGACTGCCAAGGCACACAGGGGATAGG
Num5,409,278
Avg Q17 read length78
50AQ174,442,099

Graphs

Ion Sphere™ Particle (ISP) Identification Summary

CountPercentage
Total Addressable Wells148,155,732
Wells with ISPs 130,816,028 88%
Live ISPs130,670,235 100%
Test Fragment ISPs5,776,581 4%
Library ISPs124,893,654 96%
CountPercentage
Library ISPs / Percent Enrichment124,893,654 100%
Filtered: Polyclonal66,658,574 53%
Filtered: Primer dimer277,462 <1%
Filtered: Low quality20,869,957 17%
Final Library Reads38,524,204 31%

Report Information

Analysis Info

Run Name R_2016_09_20_12_11_10_user_IONAS-304-ITLab_KN-uRNA_MV-MuSeek_160920_ITRC1-2_ITMu1-2
Run Date 2016-09-20 09:12:31+00:00
Run Flows 520
Project ITLab_ChipSeq,ITLab_ATACseq,ITLab_uRNA
Sample ITRC1a_wtBiotin
Library N/A
Reference mm10
Instrument IONAS
Flow Order TACGTACGTCTGAGCATCGATCGATGTACAGC
Library Key TCAG
TF Key ATCG
Chip Check Passed
Chip Type P1.1.17
Chip Data tiled
Notes ITLab-MVMuSeek-ATACseq_ITMu1_MV1_wtDP_bc1_ITMu2_MV2_BC16koDP_bc2-KNuRNA_ITRC1a_wtBiotin_bc5_ITRC2a_SET8koBiotin_bc6
Barcode Set MuSeek Barcode set 1
Analysis Name IONAS-304-ITLab_MV-MuSeek_160920_ITMu1-2
Analysis Date 2016-09-22
runID FQ6TW

Software Version

Torrent_Suite5.0.4
hostFW7DQV1
ion-analysis5.0.13-1
ion-chefupdates5.0.3
ion-dbreports5.0.33-1
ion-gpu5.0.0-1
ion-pipeline5.0.16-1
ion-plugins5.0.28-1
ion-protonupdates5.0.3
ion-torrentr5.0.0-1